Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women

Dobo, Cristine [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Lima, Flavio de Oliveira [UNIFESP]; Gomes, Thiago Simao [UNIFESP]; Stavale, Joao Norberto [UNIFESP]; Arias, Vitor [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Focchi, Gustavo Rubino de Azevedo [UNIFESP]

Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women

Data

2014-06-01

Autores

Dobo, Cristine

Oshima, Celina Tizuko Fujiyama

Lima, Flavio de Oliveira

Gomes, Thiago Simao

Stavale, Joao Norberto

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Tipo

Artigo

Resumo

Aim: The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21(WAF1/Cip1), significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27(Kip1), significant differences were observed between NN and GIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.000.3); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.

Citação

Anticancer Research. Athens: Int Inst Anticancer Research, v. 34, n. 6, p. 2789-2796, 2014.