Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women

Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women

Author Dobo, Cristine Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Lima, Flavio de Oliveira Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Stavale, Joao Norberto Autor UNIFESP Google Scholar
Arias, Vitor Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Focchi, Gustavo Rubino de Azevedo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Aim: The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21(WAF1/Cip1), significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27(Kip1), significant differences were observed between NN and GIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.000.3); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.
Keywords Cervical cancer
apoptosis
p53
p21(WAF1/Cip1)
p27(Kip1)
bcl-2
caspase-3
Language English
Date 2014-06-01
Published in Anticancer Research. Athens: Int Inst Anticancer Research, v. 34, n. 6, p. 2789-2796, 2014.
ISSN 0250-7005 (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 2789-2796
Origin http://ar.iiarjournals.org/content/34/6/2789.abstract
Access rights Closed access
Type Article
Web of Science ID WOS:000336875200012
URI http://repositorio.unifesp.br/11600/42602

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