Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women

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dc.contributor.authorDobo, Cristine [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorLima, Flavio de Oliveira [UNIFESP]
dc.contributor.authorGomes, Thiago Simao [UNIFESP]
dc.contributor.authorStavale, Joao Norberto [UNIFESP]
dc.contributor.authorArias, Vitor [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorFocchi, Gustavo Rubino de Azevedo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T13:50:15Z
dc.date.available2018-06-15T13:50:15Z
dc.date.issued2014-06-01
dc.description.abstractAim: The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21(WAF1/Cip1), significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27(Kip1), significant differences were observed between NN and GIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.000.3); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.en
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Dept Pathol, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Dept Pathol, BR-11060001 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent2789-2796
dc.identifierhttp://ar.iiarjournals.org/content/34/6/2789.abstract
dc.identifier.citationAnticancer Research. Athens: Int Inst Anticancer Research, v. 34, n. 6, p. 2789-2796, 2014.
dc.identifier.issn0250-7005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/42602
dc.identifier.wosWOS:000336875200012
dc.language.isoeng
dc.publisherInt Inst Anticancer Research
dc.relation.ispartofAnticancer Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCervical canceren
dc.subjectapoptosisen
dc.subjectp53en
dc.subjectp21(WAF1/Cip1)en
dc.subjectp27(Kip1)en
dc.subjectbcl-2en
dc.subjectcaspase-3en
dc.titleCell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Womenen
dc.typeinfo:eu-repo/semantics/article
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