Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
Data
2010-01-01
Autores
Sugawara, Eduardo Kinio 
Ribeiro Neto, Luciane Maria
Verreschi, Ieda Therezinha do Nascimento
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
Descrição
Citação
Química Nova. Sociedade Brasileira de Química, v. 33, n. 2, p. 447-450, 2010.