Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

Author Sugawara, Eduardo Kinio Autor UNIFESP Google Scholar
Ribeiro Neto, Luciane Maria Autor UNIFESP Google Scholar
Verreschi, Ieda Therezinha do Nascimento Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
Keywords HPLC
cortisol
urine
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 478243/2004-9
Date 2010-01-01
Published in Química Nova. Sociedade Brasileira de Química, v. 33, n. 2, p. 447-450, 2010.
ISSN 0100-4042 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Química
Extent 447-450
Origin http://dx.doi.org/10.1590/S0100-40422010000200036
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000275268400036
SciELO ID S0100-40422010000200036 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/5568

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