Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography

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dc.contributor.authorSugawara, Eduardo Kinio [UNIFESP]
dc.contributor.authorRibeiro Neto, Luciane Maria [UNIFESP]
dc.contributor.authorVerreschi, Ieda Therezinha do Nascimento [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:41:30Z
dc.date.available2015-06-14T13:41:30Z
dc.date.issued2010-01-01
dc.description.abstractDetermination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Medicina
dc.description.affiliationUnifespUNIFESP, Depto. de Medicina
dc.description.sourceSciELO
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDCNPq: 478243/2004-9
dc.format.extent447-450
dc.identifierhttp://dx.doi.org/10.1590/S0100-40422010000200036
dc.identifier.citationQuímica Nova. Sociedade Brasileira de Química, v. 33, n. 2, p. 447-450, 2010.
dc.identifier.doi10.1590/S0100-40422010000200036
dc.identifier.fileS0100-40422010000200036.pdf
dc.identifier.issn0100-4042
dc.identifier.scieloS0100-40422010000200036
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5568
dc.identifier.wosWOS:000275268400036
dc.language.isoeng
dc.publisherSociedade Brasileira de Química
dc.relation.ispartofQuímica Nova
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHPLCen
dc.subjectcortisolen
dc.subjecturineen
dc.titleDetermination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatographyen
dc.typeinfo:eu-repo/semantics/article
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