Oxigenoterapia hiperbárica em lesões actinicas de vesícula urinária de ratas: estudo histológico e histoquímico
Data
2004
Autores
Oliveira, Tânia Cilja Scheid Rodrigues de 
Orientadores
Gomes, Paulo de Oliveira
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: avaliar os efeitos da oxigeoterapia hiperbárica em lesões actínicas de vesícula
urinária de ratas submetidas a radiação ionizante. Método: foram utilizadas 38 ratas adultas
distribuídas aleatoriamente em quatro grupos: Grupo I (controle), Grupo II (radioterapia),
Grupo II (oxigenioterapia hiperbárica) e Grupo IV (radioterapia e oxigenioterapia
hiperbárica).O Grupo I foi submetido à exérese de vesícula urinária e eutanásia. O Grupo II
foi submetido a cinco sessões de oxigenoterapia hiperbárica a 3 ATA, e após três dias, exérese
de vesícula urinária e eutanásia. No Grupo III foram realizadas oito sessões de 5 Gy de
radioterapia, e após sete dias, procedimento igual a o grupo I. O grupo IV constou de oito
sessões de radioterapia, intervalo de sete dias e posterior oxigenoterapia hiperbárica, por
cinco dias e procedimento igual ao Grupo I. As vesículas urinárias foram enviadas para
estudo histológico e histoquímico. Os critérios de avaliação histopatológicos foram: necrose
da mucosa, vacuolização epitelial, necrose e atrofia da mucosa, fibrose de submucosa e
alterações vasculares. Para estudo histoquímico foi utilizado a coloração de AgNORs e a
contagem dos NORs. Resultado: não houve diferença histológica entre os grupos. As médias
de NORs encontradas foram: GI:2,1; GII:1,7; GIII:2,22 e GIV:2,21. Houve diferença
significativa do número de NORs no grupo radioterapia quando comparado aos demais
grupos. Conclusão: a radioterapia reduz a quantidade de NORs do núcleo das células, a
oxigenoterapia hiperbárica provoca recuperação do número de NORs sendo adequado o uso
do AgNOR como método de avaliação dos efeitos da radioterapia e oxigenoterapia
hiperbárica no epitélio vesical de ratos fêmeas.
Objective: avaiable the effects of the hiperbaric oxygen therapy in actinical bladder injury in female rats submitted to radiadion. Method: It was chosen 38 female rats distribuited in 4 random groups: Group I (control), Group II (radiotherapy), Group III (oxygen therapy) and Group IV (radiotherapy and hiperbaric oxygen therapy). The Group I it was submitted to bladder excise and euthanasia. The Group II it was submitted to 5 exposures to 3 ATA for 90 minutes and after 3 days surgery and euthanasia. The group III it was submitted to 8 exposures of 5 Gy each reaching a total dose of 54 Gy in bladder and after 7 days, surgery and euthanasia. The group IV it was submitted to 8 sessions of radiotherapy then hiperbaric oxygenation for 5 days and after surgery and euthanasia. The bladder species were studied by hysthologic morphology and histhochemical study. The hystologic morphologi standard it was: mucosal necrosis, epithelial vacuolization, necrosis and atrophi of mucosa, submucosal fibrosis and vascular changes. For histhochemical study to employed the AgNOR coloration and respective score of NORs. Results: There was not difference in hysthological study among the groups. The mean of NORs score it was: G I :2,1; GII: 1,7; GIII: 2,22: GIV: 2,21. There was appeared significant diference in the number of NORs in the Group II when comparable with another groups. Conclusion: the radiotherapy decrase the number of NORs, the hiperbaric oxygen therapy recovery the number of NORs and is suitable the use of AgNOR to avaiable to effects of radiation and hiperbaric oxygen therapy in the bladder of female rats.
Objective: avaiable the effects of the hiperbaric oxygen therapy in actinical bladder injury in female rats submitted to radiadion. Method: It was chosen 38 female rats distribuited in 4 random groups: Group I (control), Group II (radiotherapy), Group III (oxygen therapy) and Group IV (radiotherapy and hiperbaric oxygen therapy). The Group I it was submitted to bladder excise and euthanasia. The Group II it was submitted to 5 exposures to 3 ATA for 90 minutes and after 3 days surgery and euthanasia. The group III it was submitted to 8 exposures of 5 Gy each reaching a total dose of 54 Gy in bladder and after 7 days, surgery and euthanasia. The group IV it was submitted to 8 sessions of radiotherapy then hiperbaric oxygenation for 5 days and after surgery and euthanasia. The bladder species were studied by hysthologic morphology and histhochemical study. The hystologic morphologi standard it was: mucosal necrosis, epithelial vacuolization, necrosis and atrophi of mucosa, submucosal fibrosis and vascular changes. For histhochemical study to employed the AgNOR coloration and respective score of NORs. Results: There was not difference in hysthological study among the groups. The mean of NORs score it was: G I :2,1; GII: 1,7; GIII: 2,22: GIV: 2,21. There was appeared significant diference in the number of NORs in the Group II when comparable with another groups. Conclusion: the radiotherapy decrase the number of NORs, the hiperbaric oxygen therapy recovery the number of NORs and is suitable the use of AgNOR to avaiable to effects of radiation and hiperbaric oxygen therapy in the bladder of female rats.
Descrição
Citação
São Paulo: [s.n.], 2004. 56 p.