Artrite experimental induzida por Paracoccidioides brasiliensis em ratos
Data
2013
Autores
Loth, Eduardo Alexandre 
Orientadores
Franco, Marcello Fabiano de
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O fungo Paracoccidiodes brasiliensis (Pb) e o agente etiologico da paracoccidioidomicose (PMC), uma das mais importantes micoses sistemicas na America Latina. A PCM e a oitava causa de morte entre as doencas infecciosas e parasitarias cronicas no Brasil e apresenta a maior taxa de morte entre as micoses sistemicas. A PCM e endemica no Brasil e infecta milhares de pessoas, principalmente nos estados do Sudeste e Sul do pais. A forma pulmonar e a principal manifestacao da doenca, porem nao raramente observa-se acometimento articular, levando os individuos a sofrimento e incapacidade fisica. Objetivo: A presente pesquisa teve como objetivo desenvolver um modelo experimental de artrite fungica com o Pb em joelhos de ratos Wistar. Material e metodos: Foram utilizados ratos Wistar machos, os quais foram inoculados com 50 μl de suspensao celular de leveduras de Paracoccidioides brasiliensis nas doses de 103, 104 ou 105. Os animais foram submetidos a eutanasia aos 15 ou 45 dias apos a data da inoculacao. A articulacao do joelho direito do membro posterior de cada animal foi analisada por meio de perimetria, microscopia de luz e raios-X e amostras de sangue foram coletadas para avaliar a expressao de anticorpos anti-Pb por ELISA (enzyme-linked immunosorbent assay). Fragmentos dos orgaos abdominais foram processados para recuperacao de fungos viaveis por plaqueamento. Resultados: Os animais dos grupos submetidos a eutanasia aos 15 dias de experimento apresentaram sinais inflamatorios mais graves, maior titulacao de anticorpos anti-Pb e processo de artrite com preservacao da cartilagem articular. Os animais dos grupos com 45 dias de inoculacao desenvolveram artrite grave, com destruicao da cartilagem articular e necrose, porem sinais inflamatorios mais atenuados. Conclusoes: A metodologia utilizada foi capaz de causar artrite nos animais estudados. Aos 15 dias, observou-se processo de artrite marcado por sinovite intensa, e aos 45 dias, observou-se artrite grave. A dose de 105 celulas leveduriformes foi admitida como dose padrao para o desenvolvimento do modelo
Introduction: The fungus Paracoccidioides brasiliensis (Pb) is the etiologic agent of paracoccidioidomycosis (PMC), one of the most important systemic mycosis in Latin America. The PCM is considered the eighth most common cause of death among chronic infectious and parasitic diseases in Brazil and has the highest death rate among the systemic mycoses. The PCM is endemic in Brazil and infects thousands of people, mainly at Southeast and South States. The pulmonary form is the main manifestation of the disease, but frequently, joint involvement is observed, causing suffering and disability. Objective: This study aimed to develop an experimental model of septic arthritis with Paracoccidioides brasiliensis in the knees of rats. Material and methods: Male Wistar rats were inoculated with 50 ul of cell suspension of Pb yeast at doses of 103, 104 and 105. Animals were sacrificed 15 or 45 days after the date of inoculation. The hind limb right knee of each animal was analyzed by perimetry, light microscopy and X-ray, and blood samples were collected to assess the expression of anti-Pb by ELISA (enzyme-linked immunosorbent assay). Fragments of the abdominal organs were processed for recovery of viable fungi by plating. Results: The animals sacrificed at 15 days showed more severe signs of inflammation, higher titer of anti-Pb antibodies and arthritis with preservation of the articular cartilage. The animals with 45 days of inoculation developed severe arthritis with destruction of articular cartilage and necrosis, however inflammatory signs were more attenuated. Conclusions: The methodology was able to cause arthritis in animals studied. At 15 days, the arthritis process was marked by severe synovitis, and at 45 days, a severe arthritis was observed. A dose of 105 yeast cells was taken as standard for the experimental model.
Introduction: The fungus Paracoccidioides brasiliensis (Pb) is the etiologic agent of paracoccidioidomycosis (PMC), one of the most important systemic mycosis in Latin America. The PCM is considered the eighth most common cause of death among chronic infectious and parasitic diseases in Brazil and has the highest death rate among the systemic mycoses. The PCM is endemic in Brazil and infects thousands of people, mainly at Southeast and South States. The pulmonary form is the main manifestation of the disease, but frequently, joint involvement is observed, causing suffering and disability. Objective: This study aimed to develop an experimental model of septic arthritis with Paracoccidioides brasiliensis in the knees of rats. Material and methods: Male Wistar rats were inoculated with 50 ul of cell suspension of Pb yeast at doses of 103, 104 and 105. Animals were sacrificed 15 or 45 days after the date of inoculation. The hind limb right knee of each animal was analyzed by perimetry, light microscopy and X-ray, and blood samples were collected to assess the expression of anti-Pb by ELISA (enzyme-linked immunosorbent assay). Fragments of the abdominal organs were processed for recovery of viable fungi by plating. Results: The animals sacrificed at 15 days showed more severe signs of inflammation, higher titer of anti-Pb antibodies and arthritis with preservation of the articular cartilage. The animals with 45 days of inoculation developed severe arthritis with destruction of articular cartilage and necrosis, however inflammatory signs were more attenuated. Conclusions: The methodology was able to cause arthritis in animals studied. At 15 days, the arthritis process was marked by severe synovitis, and at 45 days, a severe arthritis was observed. A dose of 105 yeast cells was taken as standard for the experimental model.
Descrição
Citação
LOTH, Eduardo Alexandre. Artrite experimental induzida por Paracoccidioides brasiliensis em ratos.2013. 77f. Tese (Doutorado em Patologia) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2013.