Avaliação do microambiente ósseo nas metástases de carcinoma de mama e próstata
Arquivos
Data
2022-12-15
Autores
Garcia, Jairo Greco [UNIFESP]
Orientadores
Alves, Maria Teresa de Seixas [UNIFESP]
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Analisar o microambiente ósseo nas metástases de carcinoma de mama e próstata. Métodos: Foram selecionados 25 pacientes entre os sexos feminino e masculino, portadores respectivamente de carcinomas metastáticos ósseos de mama e próstata. A análise dos pacientes se deu pela avaliação imuno-histoquimica com anticorpos anti-CD105 e por hematoxilina eosina para contagem de microvasos, osteoblastos e osteoclastos no nicho metastático do microambiente ósseo. Paciente amputados por causas vasculares foram utilizados como controle do estudo. Resultados: A expressão do CD105 foi observada nos tecidos vasculares da metástase e não em tecido normal ou células do nicho tumoral. O aumento de osteoblastos e osteoclastos acompanhou o crescimento de microvasos. Em relação aos diagnósticos e carcinoma de mama e próstata não houve diferença estatística na análise dos dados quanto a localização, expressão de CD105 e densidade de microvasos, contudo, o carcinoma de mama apresentou maior contagem de osteoblastos. Conclusões: A expressão de CD105 é observada apenas nos tecidos onde ocorre a neoformação de microvasos e que o aumento destes gera um recrutamento de osteoblastos não apenas na periferia da metástase, mas também no tecido ósseo normal, bem como consequentemente o aumento do número de osteoclastos.
Objective: To analyze the bone microenvironment in breast and prostate cancer metastases. Methods: We selected 25 patients, male and female, with metastatic bone carcinomas of the breast and prostate, respectively. The patients were analyzed by immunohistochemical evaluation with anti-CD105 antibodies and hematoxylin-eosin to count microvessels, osteoblasts and osteoclasts in the metastatic niche of the bone microenvironment. Patients amputees due to vascular causes were used as study controls. Results: CD105 expression was observed in the vascular tissues of the metastasis and not in normal tissue or tumor niche cells. The increase in osteoblasts and osteoclasts accompanied the growth of microvessels. Regarding the diagnoses of breast and prostate carcinoma, there was no statistical difference in the data analysis regarding location, CD105 expression and microvessel density, however, breast carcinoma had a higher osteoblast count. Conclusions: The expression of CD105 is observed only in tissues where neoformation of microvessels occurs and that their increase generates a recruitment of osteoblasts not only in the periphery of the metastasis, but also in normal bone tissue, as well as consequently an increase in the number of osteoclasts .
Objective: To analyze the bone microenvironment in breast and prostate cancer metastases. Methods: We selected 25 patients, male and female, with metastatic bone carcinomas of the breast and prostate, respectively. The patients were analyzed by immunohistochemical evaluation with anti-CD105 antibodies and hematoxylin-eosin to count microvessels, osteoblasts and osteoclasts in the metastatic niche of the bone microenvironment. Patients amputees due to vascular causes were used as study controls. Results: CD105 expression was observed in the vascular tissues of the metastasis and not in normal tissue or tumor niche cells. The increase in osteoblasts and osteoclasts accompanied the growth of microvessels. Regarding the diagnoses of breast and prostate carcinoma, there was no statistical difference in the data analysis regarding location, CD105 expression and microvessel density, however, breast carcinoma had a higher osteoblast count. Conclusions: The expression of CD105 is observed only in tissues where neoformation of microvessels occurs and that their increase generates a recruitment of osteoblasts not only in the periphery of the metastasis, but also in normal bone tissue, as well as consequently an increase in the number of osteoclasts .