Análise da citotoxicidade e da atividade antioxidante de Baccharis trimera (Less.) DC. (Asteraceae) em queratinócitos
Data
2022-12-01
Autores
Barsalobra, Karine Pires 
Orientadores
Lopes, Patricia Santos
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Radicais livres produzidos em excesso podem causar danos à pele, tais como a perda de elasticidade e aceleração do envelhecimento. Os antioxidantes são capazes minimizar ou prevenir esses danos, reduzindo a formação de radicais livres. A Baccharis trimera (Less.) DC. (Asteraceae), conhecida popularmente como “carqueja”, possui substâncias com grande potencial antioxidante, tornando a sua investigação importante para identificar compostos naturais que possam retardar o envelhecimento cutâneo. Diante disso, este estudo teve por objetivo realizar testes in vitro em uma linhagem de queratinócitos humanos imortalizados (HaCaT) para identificar a citotoxicidade e a atividade antioxidante de extratos de B. trimera (Less.) DC. A citotoxicidade foi determinada pelo método de coloração com a solução de MTS/PMS. No ensaio de atividade antioxidante, a fluorescência foi obtida utilizando o 2’,7’–dicloroflurosceína diacetato (DCFH-DA). As substâncias isoladas e identificadas foram os flavonoides quercetina, luteolina e apigenina. Os resultados também determinaram o IC50 de 1961 μg/ml para o extrato aquoso, 460 μg/ml para o extrato hidroalcoólico, 50 μg/ml para a luteolina, 240 μg/ml para a apigenina e 241,3 μg/ml para a quercetina. As amostras testadas mostraram ótimos resultados, evidenciando um potencial antioxidante de 80,5% para o extrato aquoso, 86,0% para o extrato hidroalcoólico; 78,0% para a luteolina; 83,0% para a apigenina e 86,8% para a quercetina, indicando resultados promissores para seu uso em cosméticos, principalmente relacionados ao extrato hidroalcoólico.
Free radicals produced in excess can cause skin damage, such as loss of elasticity and accelerated aging. Antioxidants are able to minimize or prevent this damage by reducing the formation of free radicals. Baccharis trimera (Less.) DC. (Asteraceae), popularly known as “carqueja”, has substances with great antioxidant potential, making its investigation important to identify natural compounds that can delay skin aging. This study aimed to perform in vitro tests on a lineage of immortalized human keratinocytes (HaCaT) to identify the cytotoxicity and antioxidant activity of extracts of B. trimera (Less.) DC. Cytotoxicity was determined by the staining method with the MTS/PMS solution. In the antioxidant activity assay, the fluorescence was obtained using 2',7'– dichlorofluorscein diacetate (DCFHDA). The substances isolated and identified were the flavonoids quercetin, luteolin and apigenin. The results also determined IC50 of 1961 μg/ml for the aqueous extract, 460 μg/ml for the hydroalcoholic extract, 50 μg/ml for luteolin, 240 μg/ml for apigenin and 241.3 μg/ml for quercetin. The tested samples showed excellent results, showing an antioxidant potential of 80.5% for the aqueous extract, 86.0% for the hydroalcoholic extract; 78.0% for luteolin; 83.0% for apigenin and 86.8% for quercetin. The B. trimera showed promising results for its use in cosmetics, mainly related to the hydroalcoholic extract.
Free radicals produced in excess can cause skin damage, such as loss of elasticity and accelerated aging. Antioxidants are able to minimize or prevent this damage by reducing the formation of free radicals. Baccharis trimera (Less.) DC. (Asteraceae), popularly known as “carqueja”, has substances with great antioxidant potential, making its investigation important to identify natural compounds that can delay skin aging. This study aimed to perform in vitro tests on a lineage of immortalized human keratinocytes (HaCaT) to identify the cytotoxicity and antioxidant activity of extracts of B. trimera (Less.) DC. Cytotoxicity was determined by the staining method with the MTS/PMS solution. In the antioxidant activity assay, the fluorescence was obtained using 2',7'– dichlorofluorscein diacetate (DCFHDA). The substances isolated and identified were the flavonoids quercetin, luteolin and apigenin. The results also determined IC50 of 1961 μg/ml for the aqueous extract, 460 μg/ml for the hydroalcoholic extract, 50 μg/ml for luteolin, 240 μg/ml for apigenin and 241.3 μg/ml for quercetin. The tested samples showed excellent results, showing an antioxidant potential of 80.5% for the aqueous extract, 86.0% for the hydroalcoholic extract; 78.0% for luteolin; 83.0% for apigenin and 86.8% for quercetin. The B. trimera showed promising results for its use in cosmetics, mainly related to the hydroalcoholic extract.