Caracterização estrutural de bicamadas catiônicas de DODAB contendo c24:1 beta-glicosilceramida
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Data
2019-01-03
Autores
Martins, Leticia de Souza 
Nomura, Daniela Akiko
Duarte, Evandro Luiz
Riske, Karin do Amaral
Lamy, Maria Teresa Moura
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The effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 β-glucosylceramide
(βGlcCer) on the structure of cationic DODAB bilayers was investigated by means of
differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy
and fluorescence microscopy. βGlcCer is completely miscible with DODAB at all
fractions tested, since no domains were observed in fluorescence microscopy or ESR
spectra. The latter showed that βGlcCer destabilized the gel phase of DODAB bilayers
by decreasing the gel phase packing. As a consequence, βGlcCer induced a decrease in
the phase transition temperature and cooperativity o f DODAB bilayers, as seen in DSC
thermograms. ESR spectra also showed that βGlcCer induced an increase in DODAB
fluid phase order and/or rigidity. Despite their different structures, a similar effect of
loosening the gel phase packing and turning the fluid phase more rigid/organized has
also been observed when low molar fractions of cholesterol were incorporated in
DODAB bilayers. The structural characterization of mixed membranes made of cationic
2
lipids and glucosylceramides may be important for developing novel
immunotherapeutic tools such as vaccine adjuvants.