Caracterização estrutural de bicamadas catiônicas de DODAB contendo c24:1 beta-glicosilceramida

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dc.audience.educationlevel
dc.citation.issue3pt_BR
dc.citation.volume1861pt_BR
dc.contributor.authorMartins, Leticia de Souza [UNIFESP]
dc.contributor.authorNomura, Daniela Akiko [UNIFESP]
dc.contributor.authorDuarte, Evandro Luiz [UNIFESP]
dc.contributor.authorRiske, Karin do Amaral [UNIFESP]
dc.contributor.authorLamy, Maria Teresa Moura [UNIFESP]
dc.contributor.authorRozenfeld, Julio Henrique Kravcuks [UNIFESP]
dc.contributor.authorLatteshttp://lattes.cnpq.br/8580993475540515pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/5252534552656416pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/1133398377386662pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/9178927522709552pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/4052567669610380pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/4232139220639334pt_BR
dc.date.accessioned2021-06-30T11:13:41Z
dc.date.available2021-06-30T11:13:41Z
dc.date.issued2019-01-03
dc.description.abstractThe effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 β-glucosylceramide (βGlcCer) on the structure of cationic DODAB bilayers was investigated by means of differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy and fluorescence microscopy. βGlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed in fluorescence microscopy or ESR spectra. The latter showed that βGlcCer destabilized the gel phase of DODAB bilayers by decreasing the gel phase packing. As a consequence, βGlcCer induced a decrease in the phase transition temperature and cooperativity o f DODAB bilayers, as seen in DSC thermograms. ESR spectra also showed that βGlcCer induced an increase in DODAB fluid phase order and/or rigidity. Despite their different structures, a similar effect of loosening the gel phase packing and turning the fluid phase more rigid/organized has also been observed when low molar fractions of cholesterol were incorporated in DODAB bilayers. The structural characterization of mixed membranes made of cationic 2 lipids and glucosylceramides may be important for developing novel immunotherapeutic tools such as vaccine adjuvants.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFapesp 2016/19077 -1pt_BR
dc.format.extent643-650pt_BR
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0005273618303742pt_BR
dc.identifier.doi10.1016/j.bbamem.2018.12.018pt_BR
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/61231
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.relation.ispartofBBA - Biomembranespt_BR
dc.rightsinfo:eu-repo/semantics/openAccesspt_BR
dc.subjectglucosylceramidept_BR
dc.subjectdioctadecyldimethylammonium bromidept_BR
dc.subjectcationic membranept_BR
dc.subjectdifferential scanning calorimetrypt_BR
dc.subjectelectron spin resonancept_BR
dc.subjectfluorescence microscopypt_BR
dc.titleCaracterização estrutural de bicamadas catiônicas de DODAB contendo c24:1 beta-glicosilceramidapt_BR
dc.title.alternativeStructural characterization of cationic DODAB bilayers containing C24:1 β-glucosylceramideen
dc.typeinfo:eu-repo/semantics/articlept_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoBiofísicapt_BR
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