Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C

Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C

Author Lima, Lais R. Google Scholar
Moraes, Marli L. Autor UNIFESP Google Scholar
Nigoghossian, Karina Google Scholar
Peres, Maristela F. S. Google Scholar
Ribeiro, Sidney J. L. Google Scholar
Abstract Nanostructured films prepared by Layer-by-Layer technique and containing silk fibroin, antigenic peptide NS5A-1 derived from hepatitis C virus (HCV) NS5A protein and YVO4:Eu3+ luminescent nanoparticles, were utilized in sensing of hepatitis C. Detection system exploits the biorecognition between the antibody anti-HCV and the antigenic peptide NS5A-1 through changes in luminescence properties. Films deposition was monitored by UV-vis Absorption and Fluorescence Spectroscopy measurements at each bilayer deposited. The Eu3+ luminescence properties were evaluated in the presence of anti-HCV for optical detection of specific antibody and anti-HIV used as negative control. Significant changes in luminescence were observed in the presence of anti-HCV concentrations. A new immunosensor platform is proposed for optical detection of hepatitis C. (C) 2015 Elsevier B.V. All rights reserved.
Keywords Luminescent nanoparticles
Silk fibroin
Antigenic peptide
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Brazilian funding agency CNPq
Brazilian funding agency FAPESP
Nanobiotec-CAPES network (Brazil)
Date 2016
Published in Journal Of Luminescence. Amsterdam, v. 170, p. 375-379, 2016.
ISSN 0022-2313 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 375-379
Access rights Closed access
Type Article
Web of Science ID WOS:000367638500007

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