Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C

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dc.citation.volume170
dc.contributor.authorLima, Lais R.
dc.contributor.authorMoraes, Marli L. [UNIFESP]
dc.contributor.authorNigoghossian, Karina
dc.contributor.authorPeres, Maristela F. S.
dc.contributor.authorRibeiro, Sidney J. L.
dc.coverageAmsterdam
dc.date.accessioned2020-10-30T18:46:36Z
dc.date.available2020-10-30T18:46:36Z
dc.date.issued2016
dc.description.abstractNanostructured films prepared by Layer-by-Layer technique and containing silk fibroin, antigenic peptide NS5A-1 derived from hepatitis C virus (HCV) NS5A protein and YVO4:Eu3+ luminescent nanoparticles, were utilized in sensing of hepatitis C. Detection system exploits the biorecognition between the antibody anti-HCV and the antigenic peptide NS5A-1 through changes in luminescence properties. Films deposition was monitored by UV-vis Absorption and Fluorescence Spectroscopy measurements at each bilayer deposited. The Eu3+ luminescence properties were evaluated in the presence of anti-HCV for optical detection of specific antibody and anti-HIV used as negative control. Significant changes in luminescence were observed in the presence of anti-HCV concentrations. A new immunosensor platform is proposed for optical detection of hepatitis C. (C) 2015 Elsevier B.V. All rights reserved.en
dc.description.affiliationSao Paulo State Univ, Inst Chem, UNESP, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Sci & Technol, UNIFESP, BR-12231280 Sao Jose Dos Campos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Sci & Technol, UNIFESP, BR-12231280 Sao Jose Dos Campos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipBrazilian funding agency CNPq
dc.description.sponsorshipBrazilian funding agency FAPESP
dc.description.sponsorshipNanobiotec-CAPES network (Brazil)
dc.format.extent375-379
dc.identifierhttps://doi.org/10.1016/j.jlumin.2015.08.039
dc.identifier.citationJournal Of Luminescence. Amsterdam, v. 170, p. 375-379, 2016.
dc.identifier.doi10.1016/j.jlumin.2015.08.039
dc.identifier.issn0022-2313
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58529
dc.identifier.wosWOS:000367638500007
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Luminescence
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectLuminescent nanoparticlesen
dc.subjectEuropiumen
dc.subjectSilk fibroinen
dc.subjectAntigenic peptideen
dc.subjectImmunosensoren
dc.titleSilk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis Cen
dc.typeinfo:eu-repo/semantics/article
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