Facial nerve identification with fluorescent dye in rats
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Data
2016
Autores
de Melo, Giulianno Molina [UNIFESP]
Cervantes, Onivaldo [UNIFESP]
Covolan, Luciene [UNIFESP]
Baptista, Heloisa Allegro [UNIFESP]
Ferreira, Elenn Soares [UNIFESP]
Abrahao, Marcio [UNIFESP]
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PURPOSE: The parotidectomy technique still has an elevated paresis and paralysis index, lowering patient life's quality. The correct identification of the facial nerve can prevent nerve damage. Fluorescent dye identifies nerves in experimental studies but only few articles focused its use on facial nerve study in parotidectomies. We aimed to stain the rat facial nerve with fluorescent dye to facilitate visualization and dissection in order to prevent injuries. METHODS: Forty adult male Wistar rats were submitted to facial injection of saline solution (Gsf-control group, 10) or fluorescent dye solution (Gdye group, 30) followed by parotidectomy preserving the facial nerve, measuring the time for localization and facility of localization (LocTime and LFN). Nerve function was assessed using the Vibrissae Movements (PMV) and Eyelid Closure Motion (PFP) scores. RESULTS: Nerve localization was faster in Gdye group, with 83% Easy LFN rate. The Gdye group presented with low nerve injury degree and better PMV and PFP scores, with high sensitivity and accuracy. CONCLUSIONS: This experimental method of facial nerve fluorescence was effective for intraoperative nerve visualization, identification and preservation. The technique may be used in future facial nerve studies, translated to humans, contributing to the optimization of parotid surgery in the near future.
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Acta Cirurgica Brasileira. Sao Paulo, v. 31, n. 2, p. 92-102, 2016.