Facial nerve identification with fluorescent dye in rats

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dc.citation.issue2
dc.citation.volume31
dc.contributor.authorde Melo, Giulianno Molina [UNIFESP]
dc.contributor.authorCervantes, Onivaldo [UNIFESP]
dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.contributor.authorBaptista, Heloisa Allegro [UNIFESP]
dc.contributor.authorFerreira, Elenn Soares [UNIFESP]
dc.contributor.authorAbrahao, Marcio [UNIFESP]
dc.coverageSao Paulo
dc.date.accessioned2020-08-21T17:00:27Z
dc.date.available2020-08-21T17:00:27Z
dc.date.issued2016
dc.description.abstractPURPOSE: The parotidectomy technique still has an elevated paresis and paralysis index, lowering patient life's quality. The correct identification of the facial nerve can prevent nerve damage. Fluorescent dye identifies nerves in experimental studies but only few articles focused its use on facial nerve study in parotidectomies. We aimed to stain the rat facial nerve with fluorescent dye to facilitate visualization and dissection in order to prevent injuries. METHODS: Forty adult male Wistar rats were submitted to facial injection of saline solution (Gsf-control group, 10) or fluorescent dye solution (Gdye group, 30) followed by parotidectomy preserving the facial nerve, measuring the time for localization and facility of localization (LocTime and LFN). Nerve function was assessed using the Vibrissae Movements (PMV) and Eyelid Closure Motion (PFP) scores. RESULTS: Nerve localization was faster in Gdye group, with 83% Easy LFN rate. The Gdye group presented with low nerve injury degree and better PMV and PFP scores, with high sensitivity and accuracy. CONCLUSIONS: This experimental method of facial nerve fluorescence was effective for intraoperative nerve visualization, identification and preservation. The technique may be used in future facial nerve studies, translated to humans, contributing to the optimization of parotid surgery in the near future.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Otorhinolaryngol Head & Neck Surg, Head & Neck Surg Div, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Neurophysiol Lab, Dept Physiol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Expt Models Dev Ctr CEDEME, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Transgen Lab, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Neurophysiol Lab, Dept Physiol, Postgrad Program Neurosci & Neurol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Otorhinolaryngol Head & Neck Surg, Head & Neck Surg Div, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Neurophysiol Lab, Dept Physiol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Expt Models Dev Ctr CEDEME, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Transgen Lab, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Neurophysiol Lab, Dept Physiol, Postgrad Program Neurosci & Neurol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCAPES
dc.format.extent92-102
dc.identifierhttp://dx.doi.org/10.1590/S0102-865020160020000003
dc.identifier.citationActa Cirurgica Brasileira. Sao Paulo, v. 31, n. 2, p. 92-102, 2016.
dc.identifier.doi10.1590/S0102-865020160020000003
dc.identifier.fileS0102-86502016000200092-en.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502016000200092
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58002
dc.identifier.wosWOS:000372808700003
dc.language.isoeng
dc.publisherActa Cirurgica Brasileira
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFacial Nerveen
dc.subjectFluorescent Dyesen
dc.subjectParotid Glanden
dc.subjectRatsen
dc.titleFacial nerve identification with fluorescent dye in ratsen
dc.typeinfo:eu-repo/semantics/article
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