High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

Liberato, Tarcisio [UNIFESP]; Paolo Troncone, Lanfranco Ranieri; Yamashiro, Edson T.; Serrano, Solange M. T.; Zelanis, Andre [UNIFESP]

High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

Data

2016

Autores

Liberato, Tarcisio

Paolo Troncone, Lanfranco Ranieri

Yamashiro, Edson T.

Serrano, Solange M. T.

Zelanis, Andre

Tipo

Artigo

Resumo

Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.

Citação

Amino Acids. Wien, v. 48, n. 3, p. 901-906, 2016.