Late reproductive analysis in rat male offspring exposed to nicotine during pregnancy and lactation

Late reproductive analysis in rat male offspring exposed to nicotine during pregnancy and lactation

Author Miranda-Spooner, M. Autor UNIFESP Google Scholar
Paccola, C. C. Autor UNIFESP Google Scholar
Neves, F. M. O. Autor UNIFESP Google Scholar
de Oliva, S. U. Autor UNIFESP Google Scholar
Miraglia, S. M. Autor UNIFESP Google Scholar
Abstract We previously observed that nicotine, administered to rats (Wistar) during pregnancy and lactation periods, provokes, in the progeny, late morphofunctional alterations in Leydig cell, body weight increase in adulthood (90days post partum, dpp) as well as seminiferous epithelium injury. Aiming to investigate whether the spermatogenic damage previously observed in adult progenies from pregnant and lactating nicotine-exposed rat dams are maintained or whether it is worsened in older rats, we analyzed the morphological testicular alterations after up to two complete periods of spermatogenesis (53days each), spermatic parameters, and sperm DNA fragmentation. Pregnant and lactating rats were nicotine-exposed (2mg/kg/day) through an osmotic minipump implanted on the first day of pregnancy and replaced after birth. Absolute Control (no minipump) and Sham Control (minipump without nicotine) groups were established. The offspring were killed at 90, 143, and 196dpp. Significant alterations in morphometric and stereological testicular parameters, such as concentration of sperm number, daily sperm production, and plasma and intratesticular levels of cholesterol and testosterone were not observed in nicotine-exposed rats. Testicular histopathological analysis showed small intraepithelial vacuolization and an accentuated germ cell desquamation in exposed rats. However, the offspring from nicotine-exposed dams exhibited higher frequency of morphologically abnormal spermatozoa and lower sperm motility in comparison with control groups. In addition, nicotine-exposed groups showed a significant reduction in sperm mitochondrial activity and an increased sperm DNA fragmentation (Comet assay). These results indicate a late reproductive damage in the male progeny caused by maternal nicotine exposure, related to the decrease in sperm quality.
Keywords epididymis
nicotine
pregnancy
rats
spermatozoa
testis
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Coordination for the Improvement of Higher Level Personnel (CAPES/Brazil)
Date 2016
Published in Andrology. Hoboken, v. 4, n. 2, p. 218-231, 2016.
ISSN 2047-2919 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 218-231
Origin http://dx.doi.org/10.1111/andr.12100
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372417000004
URI https://repositorio.unifesp.br/handle/11600/57871

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