Late reproductive analysis in rat male offspring exposed to nicotine during pregnancy and lactation

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dc.citation.issue2
dc.citation.volume4
dc.contributor.authorMiranda-Spooner, M. [UNIFESP]
dc.contributor.authorPaccola, C. C. [UNIFESP]
dc.contributor.authorNeves, F. M. O. [UNIFESP]
dc.contributor.authorde Oliva, S. U. [UNIFESP]
dc.contributor.authorMiraglia, S. M. [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-08-21T17:00:06Z
dc.date.available2020-08-21T17:00:06Z
dc.date.issued2016
dc.description.abstractWe previously observed that nicotine, administered to rats (Wistar) during pregnancy and lactation periods, provokes, in the progeny, late morphofunctional alterations in Leydig cell, body weight increase in adulthood (90days post partum, dpp) as well as seminiferous epithelium injury. Aiming to investigate whether the spermatogenic damage previously observed in adult progenies from pregnant and lactating nicotine-exposed rat dams are maintained or whether it is worsened in older rats, we analyzed the morphological testicular alterations after up to two complete periods of spermatogenesis (53days each), spermatic parameters, and sperm DNA fragmentation. Pregnant and lactating rats were nicotine-exposed (2mg/kg/day) through an osmotic minipump implanted on the first day of pregnancy and replaced after birth. Absolute Control (no minipump) and Sham Control (minipump without nicotine) groups were established. The offspring were killed at 90, 143, and 196dpp. Significant alterations in morphometric and stereological testicular parameters, such as concentration of sperm number, daily sperm production, and plasma and intratesticular levels of cholesterol and testosterone were not observed in nicotine-exposed rats. Testicular histopathological analysis showed small intraepithelial vacuolization and an accentuated germ cell desquamation in exposed rats. However, the offspring from nicotine-exposed dams exhibited higher frequency of morphologically abnormal spermatozoa and lower sperm motility in comparison with control groups. In addition, nicotine-exposed groups showed a significant reduction in sperm mitochondrial activity and an increased sperm DNA fragmentation (Comet assay). These results indicate a late reproductive damage in the male progeny caused by maternal nicotine exposure, related to the decrease in sperm quality.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol & Genet, Dev Biol Lab, Rua Botucatu 740,Edificio Leitao Cunha,2 Andar, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Morphol & Genet, Dev Biol Lab, Rua Botucatu 740,Edificio Leitao Cunha,2 Andar, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordination for the Improvement of Higher Level Personnel (CAPES/Brazil)
dc.format.extent218-231
dc.identifierhttp://dx.doi.org/10.1111/andr.12100
dc.identifier.citationAndrology. Hoboken, v. 4, n. 2, p. 218-231, 2016.
dc.identifier.doi10.1111/andr.12100
dc.identifier.fileWOS000372417000004.pdf
dc.identifier.issn2047-2919
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57871
dc.identifier.wosWOS:000372417000004
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAndrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectepididymisen
dc.subjectnicotineen
dc.subjectpregnancyen
dc.subjectratsen
dc.subjectspermatozoaen
dc.subjecttestisen
dc.titleLate reproductive analysis in rat male offspring exposed to nicotine during pregnancy and lactationen
dc.typeinfo:eu-repo/semantics/article
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