Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.

Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.

Author Santos, Michaelle G. Google Scholar
Almeida, Valeria G. Google Scholar
Avelar-Freitas, Bethania A. Google Scholar
Grael, Cristiane F. F. Google Scholar
Gregorio, Luiz E. Autor UNIFESP Google Scholar
Pereira, Wagner F. Google Scholar
Brito-Melo, Gustavo E. A. Google Scholar
Abstract Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
Keywords Eriosema campestre
Flavonoids
Cellular proliferation
IL-2
xmlui.dri2xhtml.METS-1.0.item-coverage Curitiba-Pr
Language Portuguese
Sponsor FAPEMIG
CNPq
CAPES
Grant number FAPEMIG: CBB-APQ-00581-11
Date 2016
Published in Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016.
ISSN 0102-695X (Sherpa/Romeo, impact factor)
Publisher Soc Brasileira Farmacognosia
Extent 464-470
Origin http://dx.doi.org/10.1016/j.bjp.2015.08.009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000383732900010
SciELO ID S0102-695X2016000400464 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/57614

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