Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.

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dc.citation.issue4
dc.citation.volume26
dc.contributor.authorSantos, Michaelle G.
dc.contributor.authorAlmeida, Valeria G.
dc.contributor.authorAvelar-Freitas, Bethania A.
dc.contributor.authorGrael, Cristiane F. F.
dc.contributor.authorGregorio, Luiz E. [UNIFESP]
dc.contributor.authorPereira, Wagner F.
dc.contributor.authorBrito-Melo, Gustavo E. A.
dc.coverageCuritiba-Pr
dc.date.accessioned2020-08-14T13:44:21Z
dc.date.available2020-08-14T13:44:21Z
dc.date.issued2016
dc.description.abstractEriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.en
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Imunol, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Inst Ciencia & Tecnol, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Vales Jequitinhonha & Mucuri, Lab Farmacognosia, Diamantina, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPEMIG
dc.description.sponsorshipCNPq
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDFAPEMIG: CBB-APQ-00581-11
dc.format.extent464-470
dc.identifierhttp://dx.doi.org/10.1016/j.bjp.2015.08.009
dc.identifier.citationRevista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016.
dc.identifier.doi10.1016/j.bjp.2015.08.009
dc.identifier.fileWOS000383732900010.pdf
dc.identifier.issn0102-695X
dc.identifier.scieloS0102-695X2016000400464
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57614
dc.identifier.wosWOS:000383732900010
dc.language.isopor
dc.publisherSoc Brasileira Farmacognosia
dc.relation.ispartofRevista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEriosema campestreen
dc.subjectFlavonoidsen
dc.subjectCellular proliferationen
dc.subjectIL-2en
dc.titlePhytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.en
dc.typeinfo:eu-repo/semantics/article
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