Terpinen-4-ol, tyrosol, and beta-lapachone as potential antifungals against dimorphic fungi
Data
2016
Tipo
Artigo
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Resumo
This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and beta-lapachone against strains of Coccidioides posadasii in filamentous phase (n=22) and Histoplasma capsulatum in both filamentous (n=40) and yeast phases (n=13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 mu g/mL, 20-2860 mu g/mL, and 40-1420 mu g/mL, respectively for terpinen-4-ol
250-4000 mu g/mL, 30-2000 mu g/mL, and 10-1000 mu g/mL, respectively, for tyrosol
and 0.48-7.8 mu g/mL, 0.25-16 mu g/mL, and 0.125-4 mu g/mL, respectively for p-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
250-4000 mu g/mL, 30-2000 mu g/mL, and 10-1000 mu g/mL, respectively, for tyrosol
and 0.48-7.8 mu g/mL, 0.25-16 mu g/mL, and 0.125-4 mu g/mL, respectively for p-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Descrição
Citação
Brazilian Journal Of Microbiology. Sao Paulo, v. 47, n. 4, p. 917-924, 2016.