The chemokines secretion and the oxidative stress are targets of low-level laser therapy in allergic lung inflammation

The chemokines secretion and the oxidative stress are targets of low-level laser therapy in allergic lung inflammation

Author Carvalho, Jorge Luis Costa Autor UNIFESP Google Scholar
Aparecida de Brito, Aurileia Google Scholar
Ligeiro de Oliveira, Ana Paula Google Scholar
de Castro Faria Neto, Hugo Caire Google Scholar
Pereira, Thiago Martini Google Scholar
Carvalho, Regiane Albertini de Autor UNIFESP Google Scholar
Anatriello, Elen Autor UNIFESP Google Scholar
Aimbire, Flavio Autor UNIFESP Google Scholar
Abstract Recent studies show that low-level laser therapy (LLLT) has an important anti-inflammatory action in acute lung inflammation. The present work explored if laser therapy is able to antagonize eosinophils and allergic inflammation induced by oxidative stress in Balb/c mice. Forty-eight hours after challenge, the leukocyte counting, ROS and nitrite/nitrate level, RANTES, CCL3, CCL8 as well as eotaxins were measured in the bronchoalveolar lavage fluid (BALF) of laser-treated mice or not. Into the lung, some chemokines receptors, the iNOS activity and mRNA expression, and the activities of superoxide dismutase (SOD), catalase, gluthatione, NADPH oxidase activities and thiobarbituric acid reactive species (T-Bars) were measured. Laser-treated allergic mice presented reduction of both the ICAM-1 and eosinophil in the lungs. RANTES, CCL8, CCL3 and eotaxins were reduced in BALF of laser-treated allergic mice. In allergic mice lung LLLT decreased the CCR1 and CCR3 and restored the oxidative stress balance as well. Laser decreased the lipidic peroxidation in allergic mice lung as much as increased SOD, GPx and GR. It shows that LLLT on allergic lung inflammation involves leukocyte-attractant chemokines and endogenous antioxidant. Based on results, LLLT may ultimately become a non-invasive option in allergic lung disease treatment. [GRAPHICS] The top figure illustrates the laser decreasing the eosinophils migration into BALF and the bottom figure shows the laser upregulating the expression of heme-oxygenase (anti-oxidant enzyme) in lung tissue anti-oxidant.
Keywords asthma
eosinophils
chemokines
oxidative stress
photobiomodulation
low-level laser therapy
xmlui.dri2xhtml.METS-1.0.item-coverage Weinheim
Language English
Date 2016
Published in Journal Of Biophotonics. Weinheim, v. 9, n. 43810, p. 1208-1221, 2016.
ISSN 1864-063X (Sherpa/Romeo, impact factor)
Publisher Wiley-V C H Verlag Gmbh
Extent 1208-1221
Origin http://dx.doi.org/10.1002/jbio.201600061
Access rights Closed access
Type Article
Web of Science ID WOS:000392915800010
URI https://repositorio.unifesp.br/handle/11600/56593

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account