Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Author Bertholim, Luciana Google Scholar
Zelanis, Andre Autor UNIFESP Google Scholar
Oliveira, Ana K. Google Scholar
Serrano, Solange M. T. Google Scholar
Abstract The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs.
Keywords Snake venom
Metalloproteinase
Proteomic identification of protease cleavage sites
Peptide bond specificity
Proteome derived peptide library
HF3
xmlui.dri2xhtml.METS-1.0.item-coverage Wien
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
Grant number FAPESP: 11/16623-1
FAPESP: 13/07467-1
CAPES: 1214/2011
CNPq: 304103/2012-8
Date 2016
Published in Amino Acids. Wien, v. 48, n. 5, p. 1331-1335, 2016.
ISSN 0939-4451 (Sherpa/Romeo, impact factor)
Publisher Springer Wien
Extent 1331-1335
Origin http://dx.doi.org/10.1007/s00726-016-2218-z
Access rights Closed access
Type Article
Web of Science ID WOS:000374375300019
URI https://repositorio.unifesp.br/handle/11600/55982

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