Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase
Data
2016
Autores
Zelanis, Andre 
Tipo
Artigo
Título da Revista
ISSN da Revista
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Resumo
The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs.
Descrição
Citação
Amino Acids. Wien, v. 48, n. 5, p. 1331-1335, 2016.