Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

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dc.citation.issue5
dc.citation.volume48
dc.contributor.authorBertholim, Luciana
dc.contributor.authorZelanis, Andre [UNIFESP]
dc.contributor.authorOliveira, Ana K.
dc.contributor.authorSerrano, Solange M. T.
dc.coverageWien
dc.date.accessioned2020-07-22T13:23:00Z
dc.date.available2020-07-22T13:23:00Z
dc.date.issued2016
dc.description.abstractThe Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs.en
dc.description.affiliationInst Butantan, CeTICS, Lab Especial Toxinol Aplicada, Ctr Toxins Immune Response & Cell Signaling, Av Vital Brasil 1500, BR-05503000 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipIDFAPESP: 11/16623-1
dc.description.sponsorshipIDFAPESP: 13/07467-1
dc.description.sponsorshipIDCAPES: 1214/2011
dc.description.sponsorshipIDCNPq: 304103/2012-8
dc.format.extent1331-1335
dc.identifierhttp://dx.doi.org/10.1007/s00726-016-2218-z
dc.identifier.citationAmino Acids. Wien, v. 48, n. 5, p. 1331-1335, 2016.
dc.identifier.doi10.1007/s00726-016-2218-z
dc.identifier.issn0939-4451
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55982
dc.identifier.wosWOS:000374375300019
dc.language.isoeng
dc.publisherSpringer Wien
dc.relation.ispartofAmino Acids
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectSnake venomen
dc.subjectMetalloproteinaseen
dc.subjectProteomic identification of protease cleavage sitesen
dc.subjectPeptide bond specificityen
dc.subjectProteome derived peptide libraryen
dc.subjectHF3en
dc.titleProteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinaseen
dc.typeinfo:eu-repo/semantics/article
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