Zoanthid mucus as new source of useful biologically active proteins

Zoanthid mucus as new source of useful biologically active proteins

Author Guarnieri, Miriam Camargo Autor UNIFESP Google Scholar
Modesto, Jeanne Claine de Albuquerque Google Scholar
Perez, Carlos Daniel Google Scholar
Ottaiano, Tatiana Fontes Autor UNIFESP Google Scholar
Ferreira, Rodrigo da Silva Autor UNIFESP Google Scholar
Batista, Fabricio Pereira Autor UNIFESP Google Scholar
Brito, Marlon Vilela de Autor UNIFESP Google Scholar
Campos, Ikaro Henrique Mendes Pinto Google Scholar
Oliva, Maria Luiza Vilela Autor UNIFESP Google Scholar
Abstract Palythoa caribaeorum is a very common colonial zoanthid in the coastal reefs of Brazil. It is known for its massive production of mucus, which is traditionally used in folk medicine by fishermen in northeastern Brazil. This study identified biologically active compounds in P caribaerum mucus. Crude mucus was collected during low tides by the manual scraping of colonies

samples were maintained in an ice bath, homogenized, and centrifuged at 16,000 g for 1 hat 4 degrees C

the supernatant (mucus) was kept at -80 degrees C until use. The enzymatic (proteolytic and phospholipase A(2)), inhibitory (metallo, cysteine and serine proteases), and hemagglutinating (human erythrocyte) activities were determined. The results showed high levels of cysteine and metallo proteases, intermediate levels of phosholipase A(2), low levels of trypsin, and no elastase and chymotrypsin like activities. The mucus showed potent inhibitory activity on snake venom metalloproteases and cysteine proteinase papain. In addition, it showed agglutinating activity towards O+, B+, and A(+) erythrocyte types. The hemostatic results showed that the mucus prolongs the aPTT and PT, and strongly inhibited platelet aggregation induced by arachidonic acid, collagen, epinephrine, ADP, and thrombin. The antimicrobial activity was tested on 15 strains of bacteria and fungi through the radial diffusion assay in agar, and no activity was observed. Compounds in P. caribaeorum mucus were analyzed for the first time in this study, and our results show potential pharmacological activities in these compounds, which are relevant for use in physiopathological investigations. However, the demonstration of these activities indicates caution in the use of crude mucus in folk medicine. Furthermore, the present or absent activities identified in this mucus suggest that the studied P caribaeorum colonies were in thermal stress conditions at the time of sample collection

these conditions may precede the bleaching process in zoanthids. Hence, the use of mucus as an indicator of this process should be evaluated in the future. (C) 2018 Published by Elsevier Ltd.
Keywords Proteases
Inhibitors
Hemostasis
Cnidaria
Thermal stress
Palythoa
Metalloprotease
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor FACEPE
FAPESP
CNPq
CAPES
Grant number CAPES: AUXPE 140/2015
Date 2018
Published in Toxicon. Oxford, v. 143, p. 96-107, 2018.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Pergamon-Elsevier Science Ltd
Extent 96-107
Origin http://dx.doi.org/10.1016/j.toxicon.2018.01.012
Access rights Closed access
Type Article
Web of Science ID WOS:000426223300012
URI https://repositorio.unifesp.br/handle/11600/55921

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