Zoanthid mucus as new source of useful biologically active proteins

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dc.citation.volumev. 143
dc.contributor.authorGuarnieri, Miriam Camargo [UNIFESP]
dc.contributor.authorModesto, Jeanne Claine de Albuquerque
dc.contributor.authorPerez, Carlos Daniel
dc.contributor.authorOttaiano, Tatiana Fontes [UNIFESP]
dc.contributor.authorFerreira, Rodrigo da Silva [UNIFESP]
dc.contributor.authorBatista, Fabricio Pereira [UNIFESP]
dc.contributor.authorBrito, Marlon Vilela de [UNIFESP]
dc.contributor.authorCampos, Ikaro Henrique Mendes Pinto
dc.contributor.authorOliva, Maria Luiza Vilela [UNIFESP]
dc.coverageOxford
dc.date.accessioned2020-07-20T16:31:22Z
dc.date.available2020-07-20T16:31:22Z
dc.date.issued2018
dc.description.abstractPalythoa caribaeorum is a very common colonial zoanthid in the coastal reefs of Brazil. It is known for its massive production of mucus, which is traditionally used in folk medicine by fishermen in northeastern Brazil. This study identified biologically active compounds in P caribaerum mucus. Crude mucus was collected during low tides by the manual scraping of coloniesen
dc.description.abstractsamples were maintained in an ice bath, homogenized, and centrifuged at 16,000 g for 1 hat 4 degrees Cen
dc.description.abstractthe supernatant (mucus) was kept at -80 degrees C until use. The enzymatic (proteolytic and phospholipase A(2)), inhibitory (metallo, cysteine and serine proteases), and hemagglutinating (human erythrocyte) activities were determined. The results showed high levels of cysteine and metallo proteases, intermediate levels of phosholipase A(2), low levels of trypsin, and no elastase and chymotrypsin like activities. The mucus showed potent inhibitory activity on snake venom metalloproteases and cysteine proteinase papain. In addition, it showed agglutinating activity towards O+, B+, and A(+) erythrocyte types. The hemostatic results showed that the mucus prolongs the aPTT and PT, and strongly inhibited platelet aggregation induced by arachidonic acid, collagen, epinephrine, ADP, and thrombin. The antimicrobial activity was tested on 15 strains of bacteria and fungi through the radial diffusion assay in agar, and no activity was observed. Compounds in P. caribaeorum mucus were analyzed for the first time in this study, and our results show potential pharmacological activities in these compounds, which are relevant for use in physiopathological investigations. However, the demonstration of these activities indicates caution in the use of crude mucus in folk medicine. Furthermore, the present or absent activities identified in this mucus suggest that the studied P caribaeorum colonies were in thermal stress conditions at the time of sample collectionen
dc.description.abstractthese conditions may precede the bleaching process in zoanthids. Hence, the use of mucus as an indicator of this process should be evaluated in the future. (C) 2018 Published by Elsevier Ltd.en
dc.description.affiliationUniv Fed Pernambuco, Dept Zool, Av Prof Moraes Rego 1235,Cidade Univ, BR-50670901 Recife, PE, Brazil
dc.description.affiliationUniv Fed Pernambuco, Vitoria Acad Ctr, Rua Alto Reservatorio S-N, BR-55608680 Vitoria De Santo Antao, PE, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Biochem Dept, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Biochem Dept, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFACEPE
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipCAPES
dc.description.sponsorshipIDCAPES: AUXPE 140/2015
dc.format.extent96-107
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2018.01.012
dc.identifier.citationToxicon. Oxford, v. 143, p. 96-107, 2018.
dc.identifier.doi10.1016/j.toxicon.2018.01.012
dc.identifier.issn0041-0101
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55921
dc.identifier.wosWOS:000426223300012
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofToxicon
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectProteasesen
dc.subjectInhibitorsen
dc.subjectHemostasisen
dc.subjectCnidariaen
dc.subjectThermal stressen
dc.subjectPalythoaen
dc.subjectMetalloproteaseen
dc.titleZoanthid mucus as new source of useful biologically active proteinsen
dc.typeinfo:eu-repo/semantics/article
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