Determination of free urinary cortisol in cushing's syndrome using reversed-phase high performance liquid chromatography
Sugawara, Eduardo Kinio [UNIFESP]
Ribeiro Neto, Luciane Maria [UNIFESP]
Verreschi, Ieda Therezinha do Nascimento [UNIFESP]
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Determination of free urinary cortisol is a test of choice in the diagnosis of Cushing's syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range.
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