Characterization of PVA/glutaraldehyde hydrogels obtained using Central Composite Rotatable Design (CCRD)

Characterization of PVA/glutaraldehyde hydrogels obtained using Central Composite Rotatable Design (CCRD)

Author Morandim-Giannetti, Andreia de Araujo Google Scholar
Rubio, Samantha Regina Google Scholar
Nogueira, Regina Freitas Autor UNIFESP Google Scholar
Ortega, Fernando dos Santos Google Scholar
Magalhaes Junior, Octaviano Autor UNIFESP Google Scholar
Schor, Paulo Autor UNIFESP Google Scholar
Bersanetti, Patricia Alessandra Autor UNIFESP Google Scholar
Abstract Hydrogels are made from natural or synthetic polymers and, currently, they have many biomedical applications. In this work, the conditions for obtaining a hydrogel with similar physicochemical characteristics to the vitreous humor were defined using polyvinyl alcohol (PVA) and glutaraldehyde (GLUT) as cross-linker. The concentration of PVA and GLUT were modified, and their effect was analyzed in terms of the refractive index, density, and dynamic viscosity. The hydrogel which was obtained using 3.98% (w/V) of PVA, 3.13 mL (1.57 g) of GLUT in 100 mL, and the initial pH of 7.2 showed similar characteristics to the vitreous humor (density= 1.0174 +/- 0.0050 g mL(-1), dynamic viscosity=3.7425 +/- 0.1800 mPa s and refractive index=1.3410 +/- 0.0010). The hydrogels were further investigated by rheological measurements, infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and determination of swelling degree. The reticulation with GLUT promoted an increase in viscosity and glass transition temperature. On the other hand, it stimulated a decrease in the swelling degree, crystallinity, melting temperature, and intensity of the band related to the -OH bond, compared with the PVA without reticulation. The reticulated hydrogel displayed Newtonian behavior and a higher apparent viscosity than the PVA. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1558-1566, 2018.
Keywords design of experiments
vitreous humor
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Sao Paulo Research Foundation (FAPESP)
Centro Universitario FEI
Grant number FAPESP: 2012/07343-8
Date 2018
Published in Journal Of Biomedical Materials Research Part B-Applied Biomaterials. Hoboken, v. 106, n. 4, p. 1558-1566, 2018.
ISSN 1552-4973 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 1558-1566
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000430123100015

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