Antibacterial activity of nitric oxide releasing silver nanoparticles

Antibacterial activity of nitric oxide releasing silver nanoparticles

Author Seabra, Amedea B. Autor UNIFESP Google Scholar
Manosalva, Nixson Google Scholar
Lima, Bruna de Araujo Google Scholar
Pelegrino, Milena T. Autor UNIFESP Google Scholar
Brocchi, Marcelo Google Scholar
Rubilar, Olga Google Scholar
Duran, Nelson Google Scholar
Abstract Silver nanoparticles (AgNPs) are well known potent antimicrobial agents. Similarly, the free radical nitric oxide (NO) has important antibacterial activity, and due to its instability, the combination of NO and nanomaterials has been applied in several biomedical applications. The aim of this work was to synthesize, characterize and evaluate the antibacterial activity of a new NO-releasing AgNPs. Herein, AgNPs were synthesized by the reduction of silver ions (Ag+) by catechin, a natural polyphenol and potent antioxidant agent, derived from green tea extract. Catechin acts as a reducing agent and as a capping molecule on the surface of AgNPs, minimizing particle agglomeration. The as-synthesized nanoparticles were characterized by different techniques. The results showed the formation of AgNPs with average hydrodynamic size of 44 nm, polydispersity index of 0.21, and zeta potential of -35.9 mV. X-ray diffraction and Fourier transform infrared spectroscopy revealed the presence of the AgNP core and cathecin as capping agent. The low molecular weight mercaptosuccinic acid (MSA), which contain free thiol group, was added on the surface of catechin-AgNPs, leading to the formation of MSA-catechin-AgNPs (the NO precursor nanoparticle). Free thiol groups of MSA-catechin-AgNPs were nitrosated leading to the formation of S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), the NO donor. The amount of 342 +/- 16 mu mol of NO was released per gram of S-nitroso-MSA-catechin-AgNPs. The antibacterial activities of catechin-AgNPs, MSA-catechinAgNPs, and S-nitroso-MSA-catechin-AgNPs were evaluated towards different resistant bacterial strains. The results demonstrated an enhanced antibacterial activity of the NO-releasing AgNP. For instance, the minimal inhibitory concentration values for Pseudomonas aeruginosa (ATCC 27853) incubated with AgNPs-catechin, AgNPs-catechin-MSA, and AgNPs-catechin-S-nitroso-MSA were found to be 62, 125 and 3 mu g/mL, respectively. While in the case of Klebsiella pneumoniae (ATCC 700603) the minimum bactericidal concentration values for treatments with AgNPs-catechin, AgNPs-catechin-MSA, and AgNPs-catechin-Snitroso- MSA were found to be 1000, 500, and 125 mu g/mL, respectively. The antibacterial actions of the NO-releasing nanoparticle were superior in comparison with the antibacterial effects of AgNPs, in most of the tested antibiotic resistant bacteria strains. These results highlight the promising uses of NO-releasing AgNPs against resistant bacteria in several biomedical applications.
xmlui.dri2xhtml.METS-1.0.item-coverage Bristol
Language English
Sponsor Brazilian Network on Nanotoxicology (MCTI/CNPq)
Laboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI)
FONDECYT
CONICYT REDES
Grant number Brazilian Network on Nanotoxicology (MCTI/CNPq): 552120/2011-1
Laboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI): 402280-2013
FONDECYT: 1130854
CONICYT REDES: 140053
Date 2017
Published in 5th Nanosafe International Conference On Health And Safety Issues Related To Nanomaterials For A Socially Responsible Approach (Nanosafe 2016). Bristol, v. 838, p. -, 2017.
ISSN 1742-6588 (Sherpa/Romeo, impact factor)
Publisher Iop Publishing Ltd
Extent -
Origin http://dx.doi.org/10.1088/1742-6596/838/1/012031
Access rights Open access Open Access
Type Conference paper
Web of Science ID WOS:000409540400031
URI https://repositorio.unifesp.br/handle/11600/55472

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