Antibacterial activity of nitric oxide releasing silver nanoparticles

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dc.citation.volume838
dc.contributor.authorSeabra, Amedea B. [UNIFESP]
dc.contributor.authorManosalva, Nixson
dc.contributor.authorLima, Bruna de Araujo
dc.contributor.authorPelegrino, Milena T. [UNIFESP]
dc.contributor.authorBrocchi, Marcelo
dc.contributor.authorRubilar, Olga
dc.contributor.authorDuran, Nelson
dc.coverageBristol
dc.date.accessioned2020-07-17T14:03:32Z
dc.date.available2020-07-17T14:03:32Z
dc.date.issued2017
dc.description.abstractSilver nanoparticles (AgNPs) are well known potent antimicrobial agents. Similarly, the free radical nitric oxide (NO) has important antibacterial activity, and due to its instability, the combination of NO and nanomaterials has been applied in several biomedical applications. The aim of this work was to synthesize, characterize and evaluate the antibacterial activity of a new NO-releasing AgNPs. Herein, AgNPs were synthesized by the reduction of silver ions (Ag+) by catechin, a natural polyphenol and potent antioxidant agent, derived from green tea extract. Catechin acts as a reducing agent and as a capping molecule on the surface of AgNPs, minimizing particle agglomeration. The as-synthesized nanoparticles were characterized by different techniques. The results showed the formation of AgNPs with average hydrodynamic size of 44 nm, polydispersity index of 0.21, and zeta potential of -35.9 mV. X-ray diffraction and Fourier transform infrared spectroscopy revealed the presence of the AgNP core and cathecin as capping agent. The low molecular weight mercaptosuccinic acid (MSA), which contain free thiol group, was added on the surface of catechin-AgNPs, leading to the formation of MSA-catechin-AgNPs (the NO precursor nanoparticle). Free thiol groups of MSA-catechin-AgNPs were nitrosated leading to the formation of S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), the NO donor. The amount of 342 +/- 16 mu mol of NO was released per gram of S-nitroso-MSA-catechin-AgNPs. The antibacterial activities of catechin-AgNPs, MSA-catechinAgNPs, and S-nitroso-MSA-catechin-AgNPs were evaluated towards different resistant bacterial strains. The results demonstrated an enhanced antibacterial activity of the NO-releasing AgNP. For instance, the minimal inhibitory concentration values for Pseudomonas aeruginosa (ATCC 27853) incubated with AgNPs-catechin, AgNPs-catechin-MSA, and AgNPs-catechin-S-nitroso-MSA were found to be 62, 125 and 3 mu g/mL, respectively. While in the case of Klebsiella pneumoniae (ATCC 700603) the minimum bactericidal concentration values for treatments with AgNPs-catechin, AgNPs-catechin-MSA, and AgNPs-catechin-Snitroso- MSA were found to be 1000, 500, and 125 mu g/mL, respectively. The antibacterial actions of the NO-releasing nanoparticle were superior in comparison with the antibacterial effects of AgNPs, in most of the tested antibiotic resistant bacteria strains. These results highlight the promising uses of NO-releasing AgNPs against resistant bacteria in several biomedical applications.en
dc.description.affiliationUniv Fed ABC, Ctr Nat & Human Sci, Santo Andre, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Exact & Earth Sci Dept, Diadema, SP, Brazil
dc.description.affiliationUniv La Frontera, Chem Engn Dept, Temuco, Chile
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Trop Dis Lab, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Biol Chem Lab, Inst Chem, Campinas, SP, Brazil
dc.description.affiliationLNNano CNPEM, Campinas, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Exact & Earth Sci Dept, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipBrazilian Network on Nanotoxicology (MCTI/CNPq)
dc.description.sponsorshipLaboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI)
dc.description.sponsorshipFONDECYT
dc.description.sponsorshipCONICYT REDES
dc.description.sponsorshipIDBrazilian Network on Nanotoxicology (MCTI/CNPq): 552120/2011-1
dc.description.sponsorshipIDLaboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI): 402280-2013
dc.description.sponsorshipIDFONDECYT: 1130854
dc.description.sponsorshipIDCONICYT REDES: 140053
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1088/1742-6596/838/1/012031
dc.identifier.citation5th Nanosafe International Conference On Health And Safety Issues Related To Nanomaterials For A Socially Responsible Approach (Nanosafe 2016). Bristol, v. 838, p. -, 2017.
dc.identifier.doi10.1088/1742-6596/838/1/012031
dc.identifier.fileWOS000409540400031.pdf
dc.identifier.issn1742-6588
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55472
dc.identifier.wosWOS:000409540400031
dc.language.isoeng
dc.publisherIop Publishing Ltd
dc.relation.ispartof5th Nanosafe International Conference on Health and Safety Issues Related to Nanomaterials for a Socially Responsible Approach (NANOSAFE)
dc.rightsAcesso aberto
dc.titleAntibacterial activity of nitric oxide releasing silver nanoparticlesen
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