Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin

Date
2017Author
Scaffa, Polliana Mendes Candia
Breschi, Lorenzo
Mazzoni, Annalisa
Vidal, Cristina de Mattos Pimenta
Curci, Rosa
Apolonio, Fabianni
Gobbi, Pietro
Pashley, David
Tjaderhane, Leo
Tersariol, Ivarne Luis dos Santos [UNIFESP]
Nascimento, Fabio Dupart
Carrilhon, Marcela Rocha
Type
ArtigoISSN
0003-9969Is part of
Archives Of Oral BiologyDOI
10.1016/j.archoralbio.2016.11.011Metadata
Show full item recordAbstract
It has been hypothesized that cysteine cathepsins (CTs) along with matrix metalloproteases (MMPs) may work in conjunction in the proteolysis of mature dentin matrix. The aim of this study was to verify simultaneously the distribution and presence of cathepsins B (CT-B) and K (CT-K) in partially demineralized dentin; and further to evaluate the activity of CTs and MMPs in the same tissue. The distribution of CT-B and CT-K in sound human dentin was assessed by immunohistochemistry. A double-immunolabeling technique was used to identify, at once, the occurrence of those enzymes in dentin. Activities of CTs and MMPs in dentin extracts were evaluated spectrofluorometrically. In addition, in situ gelatinolytic activity of dentin was assayed by zymography. The results revealed the distribution of CT-B and CT-K along the dentin organic matrix and also indicated co-occurrence of MMPs and CTs in that tissue. The enzyme kinetics studies showed proteolytic activity in dentin extracts for both classes of proteases. Furthermore, it was observed that, at least for sound human dentin matrices, the activity of MMPs seems to be predominant over the CTs one.
Citation
Archives Of Oral Biology. Oxford, v. 74, p. 101-107, 2017.Keywords
Cysteine cathepsinsMatrix metalloproteases
Proteolytic activity
Dentin
Immunohistochemistry
Sponsorship
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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