Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin

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dc.citation.volume74
dc.contributor.authorScaffa, Polliana Mendes Candia
dc.contributor.authorBreschi, Lorenzo
dc.contributor.authorMazzoni, Annalisa
dc.contributor.authorVidal, Cristina de Mattos Pimenta
dc.contributor.authorCurci, Rosa
dc.contributor.authorApolonio, Fabianni
dc.contributor.authorGobbi, Pietro
dc.contributor.authorPashley, David
dc.contributor.authorTjaderhane, Leo
dc.contributor.authorTersariol, Ivarne Luis dos Santos [UNIFESP]
dc.contributor.authorNascimento, Fabio Dupart
dc.contributor.authorCarrilhon, Marcela Rocha
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageOxford
dc.date.accessioned2020-07-17T14:03:10Z
dc.date.available2020-07-17T14:03:10Z
dc.date.issued2017
dc.description.abstractIt has been hypothesized that cysteine cathepsins (CTs) along with matrix metalloproteases (MMPs) may work in conjunction in the proteolysis of mature dentin matrix. The aim of this study was to verify simultaneously the distribution and presence of cathepsins B (CT-B) and K (CT-K) in partially demineralized dentin; and further to evaluate the activity of CTs and MMPs in the same tissue. The distribution of CT-B and CT-K in sound human dentin was assessed by immunohistochemistry. A double-immunolabeling technique was used to identify, at once, the occurrence of those enzymes in dentin. Activities of CTs and MMPs in dentin extracts were evaluated spectrofluorometrically. In addition, in situ gelatinolytic activity of dentin was assayed by zymography. The results revealed the distribution of CT-B and CT-K along the dentin organic matrix and also indicated co-occurrence of MMPs and CTs in that tissue. The enzyme kinetics studies showed proteolytic activity in dentin extracts for both classes of proteases. Furthermore, it was observed that, at least for sound human dentin matrices, the activity of MMPs seems to be predominant over the CTs one.en
dc.description.affiliationUniv Estadual Campinas, Fac Dent Piracicaba, BR-13414903 Piracicaba, Brazil
dc.description.affiliationUniv Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP, Brazil
dc.description.affiliationUniv Bologna, Dept Biomed & Neuromotor Sci, I-40126 Bologna, Italy
dc.description.affiliationUniv Trieste, Dept Med Sci, I-34128 Trieste, Italy
dc.description.affiliationUniv Iowa, Coll Dent, Dept Operat Dent, 801 Newton Rd, Iowa City, IA 52246 USA
dc.description.affiliationCNR, IGM, Unit Bologna, IOR, I-40136 Bologna, Italy
dc.description.affiliationUniv Fed Ceara, Dept Pharm Dent & Nursing, BR-60020181 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Carlo Bo, Dept SteVA, I-61029 Urbino, Italy
dc.description.affiliationGeorgia Hlth Sci Univ, Dent Coll Georgia, Oral Biol, Augusta, GA 30912 USA
dc.description.affiliationUniv Oulu, Inst Dent, FI-90014 Oulu, Finland
dc.description.affiliationOulu Univ Hosp, Med Res Ctr Oulu, FI-90014 Oulu, Finland
dc.description.affiliationUniv Oulu, FI-90014 Oulu, Finland
dc.description.affiliationUniv Helsinki, Dept Oral & Maxillofacial Dis, FI-00014 Helsinki, Finland
dc.description.affiliationHelsinki Univ Hosp, FI-00014 Helsinki, Finland
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, BR-04021001 Sao Paulo, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, BR-08773520 Mogi Das Cruzes, Brazil
dc.description.affiliationAnhanguera Univ Sao Paulo UNIAN SP, Biomat Dent Program, BR-05145200 Sao Paulo, Brazil
dc.description.affiliationAnhanguera Univ Sao Paulo UNIAN SP, Biotechnol & Innovat Hlth Program, BR-05145200 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, BR-04021001 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipIDFAPESP: 2007/54618-4
dc.description.sponsorshipIDFAPESP: 2009/14005-9
dc.description.sponsorshipIDCNPq: 312768/2013-3
dc.format.extent101-107
dc.identifierhttps://dx.doi.org/10.1016/j.archoralbio.2016.11.011
dc.identifier.citationArchives Of Oral Biology. Oxford, v. 74, p. 101-107, 2017.
dc.identifier.doi10.1016/j.archoralbio.2016.11.011
dc.identifier.issn0003-9969
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55204
dc.identifier.wosWOS:000393017400015
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofArchives Of Oral Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCysteine cathepsinsen
dc.subjectMatrix metalloproteasesen
dc.subjectProteolytic activityen
dc.subjectDentinen
dc.subjectImmunohistochemistryen
dc.titleCo-distribution of cysteine cathepsins and matrix metalloproteases in human dentinen
dc.typeinfo:eu-repo/semantics/article
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