Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells

Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells

Author Bryant, L. Henry, Jr. Google Scholar
Kim, Saejeong J. Google Scholar
Hobson, Matthew Google Scholar
Milo, Blerta Google Scholar
Kovacs, Zsofia I. Google Scholar
Jikaria, Neekita Google Scholar
Lewis, Bobbi K. Google Scholar
Aronova, Maria A. Google Scholar
Sousa, Alioscka A. {UNIFESP} Google Scholar
Zhang, Guofeng Google Scholar
Leapman, Richard D. Google Scholar
Frank, Joseph A. Google Scholar
Abstract Stem cell-based therapies have become a major focus in regenerative medicine and to treat diseases. A straightforward approach combining three drugs, heparin (H), protamine (P) with ferumoxytol (F) in the form of nanocomplexes (NCs) effectively labeled stem cells for cellular MRI. We report on the physicochemical characteristics for optimizing the H, P, and F components in different ratios, and mixing sequences, producing NCs that varied in hydrodynamic size. NC size depended on the order in which drugs were mixed in media. Electron microscopy of HPF or FHP showed that F was located on the surface of spheroidal shaped HP complexes. Human stem cells incubated with FHP NCs resulted in a significantly greater iron concentration per cell compared to that found in HPF NCs with the same concentration of F. These results indicate that FHP could be useful for labeling stem cells in translational studies in the clinic. Published by Elsevier Inc.
Keywords Ferumoxytol
Heparin
Protamine
Cell labeling
Stem cells
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Intramural Research Program of the Clinical Center at the National Institutes of Health
Intramural Research Program of the National Institute of Biomedical Imaging and Bioengineering at the National Institutes of Health
Date 2017
Published in Nanomedicine-Nanotechnology Biology And Medicine. Amsterdam, v. 13, n. 2, p. 503-513, 2017.
ISSN 1549-9634 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 503-513
Origin http://dx.doi.org/10.1016/j.nano.2016.07.011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000396940200016
URI https://repositorio.unifesp.br/handle/11600/55174

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