Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells

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dc.citation.issue2
dc.citation.volume13
dc.contributor.authorBryant, L. Henry, Jr.
dc.contributor.authorKim, Saejeong J.
dc.contributor.authorHobson, Matthew
dc.contributor.authorMilo, Blerta
dc.contributor.authorKovacs, Zsofia I.
dc.contributor.authorJikaria, Neekita
dc.contributor.authorLewis, Bobbi K.
dc.contributor.authorAronova, Maria A.
dc.contributor.authorSousa, Alioscka A. {UNIFESP}
dc.contributor.authorZhang, Guofeng
dc.contributor.authorLeapman, Richard D.
dc.contributor.authorFrank, Joseph A.
dc.coverageAmsterdam
dc.date.accessioned2020-07-17T14:03:07Z
dc.date.available2020-07-17T14:03:07Z
dc.date.issued2017
dc.description.abstractStem cell-based therapies have become a major focus in regenerative medicine and to treat diseases. A straightforward approach combining three drugs, heparin (H), protamine (P) with ferumoxytol (F) in the form of nanocomplexes (NCs) effectively labeled stem cells for cellular MRI. We report on the physicochemical characteristics for optimizing the H, P, and F components in different ratios, and mixing sequences, producing NCs that varied in hydrodynamic size. NC size depended on the order in which drugs were mixed in media. Electron microscopy of HPF or FHP showed that F was located on the surface of spheroidal shaped HP complexes. Human stem cells incubated with FHP NCs resulted in a significantly greater iron concentration per cell compared to that found in HPF NCs with the same concentration of F. These results indicate that FHP could be useful for labeling stem cells in translational studies in the clinic. Published by Elsevier Inc.en
dc.description.affiliationCtr Clin, Lab Diagnost Radiol Res Radiol & Imaging Sci, Bethesda, MD USA
dc.description.affiliationNIH, Frank Lab Radiol & Imaging Sci, Ctr Clin, Bethesda, MD 20892 USA
dc.description.affiliationNatl Inst Biomed Imaging & Bioengn, Lab Cellular Imaging & Macromol Biophys, NIH, Bethesda, MD USA
dc.description.affiliationNatl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipIntramural Research Program of the Clinical Center at the National Institutes of Health
dc.description.sponsorshipIntramural Research Program of the National Institute of Biomedical Imaging and Bioengineering at the National Institutes of Health
dc.format.extent503-513
dc.identifierhttp://dx.doi.org/10.1016/j.nano.2016.07.011
dc.identifier.citationNanomedicine-Nanotechnology Biology And Medicine. Amsterdam, v. 13, n. 2, p. 503-513, 2017.
dc.identifier.doi10.1016/j.nano.2016.07.011
dc.identifier.issn1549-9634
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55174
dc.identifier.wosWOS:000396940200016
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofNanomedicine-Nanotechnology Biology And Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFerumoxytolen
dc.subjectHeparinen
dc.subjectProtamineen
dc.subjectCell labelingen
dc.subjectStem cellsen
dc.titlePhysicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cellsen
dc.typeinfo:eu-repo/semantics/article
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