Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant

Author Luciano Gomes, Rudnei de Oliveira Autor UNIFESP Google Scholar
Neto, Ricardo Artigiani Autor UNIFESP Google Scholar
Guimaraes Neto, Jose de Freitas Autor UNIFESP Google Scholar
Nunes, Adriana Porto Autor UNIFESP Google Scholar
de Souza Montero, Edna Frasson Autor UNIFESP Google Scholar
Martins, Jose Luiz Autor UNIFESP Google Scholar
Abstract Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention

2) Hypoxia-Reoxygenation (H/R) - Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation

3) Remote Ischemic Preconditioning (r-IPC) - newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation. Segments of ileum were prepared for histological analysis by HE and immunohistochemistry by the Ki67 to evaluate cell proliferation, crypt depth and villus height and evaluation of apoptosis by cleaved caspase-3. Results: The intensity of the lesions was lower in the r-IPC than in the H/R group, showing significant difference (p<0.01). The r-IPC group showed a higher proliferative activity compared to the H/R group (p<0.01), with deeper crypts (r-IPC > H/R - p < 0.05), and higher villi, showing significant difference (r-IPC > H/R - (p < 0.01). The occurrence of apoptosis in the H/R group was lower in comparison to groups C and r-IPC, with significant difference (H/R < r-IPC

p<0.05). Conclusion: The remote ischemic preconditioning applied to the pregnant rat protected the ileum of newborn rats subjected to hypoxia and reoxygenation, with decreased intensity of the lesions in the ileum mucosa and preservation of proliferative activity, keeping the villus height and crypt depth similar to group C.
Keywords Enterocolitis, Necrotizing
Ischemic Preconditioning
Pregnancy, Animal
Rats
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Date 2017
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 3, p. 236-242, 2017.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 236-242
Origin http://dx.doi.org/10.1590/S0102-865020170030000008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000399082900008
URI https://repositorio.unifesp.br/handle/11600/54981

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