Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms

Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms

Author Macedo, Silene Autor UNIFESP Google Scholar
Ferreira Rodrigues, Joao Paulo Autor UNIFESP Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Yoshida, Nobuko Autor UNIFESP Google Scholar
Abstract The involvement of beta-adrenergic receptor (beta-AR) in host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is not known. We examined whether isoproterenol, an agonist of beta-AR, or nonselective beta-blocker propranolol affected MT internalization mediated the stage-specific surface molecule gp82. Treatment of HeLa cells with propranolol significantly inhibited MT invasion whereas isoproterenol had no effect. Propranolol, but not isoproterenol, also inhibited the lysosome spreading required for gp82-dependent MT invasion. The effect of propranolol in inhibiting MT internalization was not due to the prevention of gp82 interaction with beta-AR. It was mainly associated with its ability to impair lysosome spreading. (C) 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords Trypanosoma cruzi
Metacyclic trypomastigote
Propranolol
Host cell invasion
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/51475-3]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [303825/2015-4]
Date 2017
Published in Microbes And Infection. Amsterdam, v. 19, p. 295-301, 2017.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 295-301
Origin http://dx.doi.org/10.1016/j.micinf.2017.01.004
Access rights Closed access
Type Article
Web of Science ID WOS:000401213500009
URI https://repositorio.unifesp.br/handle/11600/54797

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