Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms

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dc.citation.volume19
dc.contributor.authorMacedo, Silene [UNIFESP]
dc.contributor.authorFerreira Rodrigues, Joao Paulo [UNIFESP]
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.authorYoshida, Nobuko [UNIFESP]
dc.coverageAmsterdam
dc.date.accessioned2020-07-17T14:02:25Z
dc.date.available2020-07-17T14:02:25Z
dc.date.issued2017
dc.description.abstractThe involvement of beta-adrenergic receptor (beta-AR) in host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is not known. We examined whether isoproterenol, an agonist of beta-AR, or nonselective beta-blocker propranolol affected MT internalization mediated the stage-specific surface molecule gp82. Treatment of HeLa cells with propranolol significantly inhibited MT invasion whereas isoproterenol had no effect. Propranolol, but not isoproterenol, also inhibited the lysosome spreading required for gp82-dependent MT invasion. The effect of propranolol in inhibiting MT internalization was not due to the prevention of gp82 interaction with beta-AR. It was mainly associated with its ability to impair lysosome spreading. (C) 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, 669-6 Andar, BR-04039032 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, 669-6 Andar, BR-04039032 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/51475-3]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [303825/2015-4]
dc.format.extent295-301
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2017.01.004
dc.identifier.citationMicrobes And Infection. Amsterdam, v. 19, p. 295-301, 2017.
dc.identifier.doi10.1016/j.micinf.2017.01.004
dc.identifier.issn1286-4579
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54797
dc.identifier.wosWOS:000401213500009
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofMicrobes And Infection
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectTrypanosoma cruzien
dc.subjectMetacyclic trypomastigoteen
dc.subjectPropranololen
dc.subjectHost cell invasionen
dc.titleBeta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic formsen
dc.typeinfo:eu-repo/semantics/article
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