High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates
Data
2017
Autores
Passos-Castilho, Ana Maria 
Hernandes Granato, Celso Francisco
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Parana State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus RNA was detected in 34 (20.0%) of the 170 pigs following positive results in at least one set of screening real-time RT-PCR primers and probes. The swine hepatitis E virus strains clustered with the genotype hepatitis E virus-3b reference sequences in the phylogenetic analysis and showed close similarity to human hepatitis E virus isolates previously reported in Brazil. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Descrição
Citação
Brazilian Journal Of Microbiology. Sao Paulo, v. 48, n. 2, p. 373-379, 2017.