High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates

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dc.citation.issue2
dc.citation.volume48
dc.contributor.authorPassos-Castilho, Ana Maria [UNIFESP]
dc.contributor.authorHernandes Granato, Celso Francisco [UNIFESP]
dc.coverageSao Paulo
dc.date.accessioned2020-07-17T14:02:24Z
dc.date.available2020-07-17T14:02:24Z
dc.date.issued2017
dc.description.abstractHepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Parana State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus RNA was detected in 34 (20.0%) of the 170 pigs following positive results in at least one set of screening real-time RT-PCR primers and probes. The swine hepatitis E virus strains clustered with the genotype hepatitis E virus-3b reference sequences in the phylogenetic analysis and showed close similarity to human hepatitis E virus isolates previously reported in Brazil. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Med, Div Doencas Infecciosas, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Med, Div Doencas Infecciosas, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo [2012/22925-3, 2013.03701-0]
dc.format.extent373-379
dc.identifierhttp://dx.doi.org/10.1016/j.bjm.2016.10.022
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo, v. 48, n. 2, p. 373-379, 2017.
dc.identifier.doi10.1016/j.bjm.2016.10.022
dc.identifier.issn1517-8382
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54791
dc.identifier.wosWOS:000401456800029
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBrazilen
dc.subjectGenotype 3en
dc.subjectHepatitis E virusen
dc.subjectPigsen
dc.subjectZoonosisen
dc.titleHigh frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolatesen
dc.typeinfo:eu-repo/semantics/article
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