Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death

Date
2017Author
Silva, Suelen Feitoza
Levy, Debora
Maria Ruiz, Jorge Luis
de Melo, Thatiana Correa
Isaac, Cesar
Fidelis, Maira Luisa [UNIFESP]
Rodrigues, Alessandro [UNIFESP]
Bydlowski, Sergio Paulo
Type
ArtigoISSN
0960-0760Is part of
Journal Of Steroid Biochemistry And Molecular BiologyDOI
10.1016/j.jsbmb.2016.04.017Metadata
Show full item recordAbstract
Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24 h) cytotoxic effects of cholestan-3 alpha-5 beta-6 alpha-triol, 3,5 cholestan-7-one, (3 alpha-5 beta-6 alpha)- cholestane-3,6-diol, 7-oxocholest-5-en-3 beta-ylacetate, and 5 beta-6 beta epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3 beta-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3 alpha-5 beta-6 alpha-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed. (C) 2016 Elsevier Ltd. All rights reserved.
Citation
Journal Of Steroid Biochemistry And Molecular Biology. Oxford, v. 169, p. 164-175, 2017.Keywords
OxysterolMesenchymal stem cell
Apoptosis
Cell death
Mitochondrial hyperpolarization
Adipose tissue
Sponsorship
Financiadora de Estudos e Projetos (FINEP), BrazilConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil
Institute Nacional de Ciencia e Tecnologia - Fluidos Complexos (INCT-FCx), Brazil
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