Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death

Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death

Author Silva, Suelen Feitoza Google Scholar
Levy, Debora Google Scholar
Maria Ruiz, Jorge Luis Google Scholar
de Melo, Thatiana Correa Google Scholar
Isaac, Cesar Google Scholar
Fidelis, Maira Luisa Autor UNIFESP Google Scholar
Rodrigues, Alessandro Autor UNIFESP Google Scholar
Bydlowski, Sergio Paulo Google Scholar
Abstract Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24 h) cytotoxic effects of cholestan-3 alpha-5 beta-6 alpha-triol, 3,5 cholestan-7-one, (3 alpha-5 beta-6 alpha)- cholestane-3,6-diol, 7-oxocholest-5-en-3 beta-ylacetate, and 5 beta-6 beta epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3 beta-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3 alpha-5 beta-6 alpha-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed. (C) 2016 Elsevier Ltd. All rights reserved.
Keywords Oxysterol
Mesenchymal stem cell
Cell death
Mitochondrial hyperpolarization
Adipose tissue
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor Financiadora de Estudos e Projetos (FINEP), Brazil
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil
Institute Nacional de Ciencia e Tecnologia - Fluidos Complexos (INCT-FCx), Brazil
Date 2017
Published in Journal Of Steroid Biochemistry And Molecular Biology. Oxford, v. 169, p. 164-175, 2017.
ISSN 0960-0760 (Sherpa/Romeo, impact factor)
Publisher Pergamon-Elsevier Science Ltd
Extent 164-175
Origin http://dx.doi.org/10.1016/j.jsbmb.2016.04.017
Access rights Closed access
Type Article
Web of Science ID WOS:000401391300019
URI https://repositorio.unifesp.br/handle/11600/54604

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