Author |
Silva, Suelen Feitoza
![]() Levy, Debora ![]() Maria Ruiz, Jorge Luis ![]() de Melo, Thatiana Correa ![]() Isaac, Cesar ![]() Fidelis, Maira Luisa ![]() ![]() Rodrigues, Alessandro ![]() ![]() Bydlowski, Sergio Paulo ![]() |
Abstract | Mesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24 h) cytotoxic effects of cholestan-3 alpha-5 beta-6 alpha-triol, 3,5 cholestan-7-one, (3 alpha-5 beta-6 alpha)- cholestane-3,6-diol, 7-oxocholest-5-en-3 beta-ylacetate, and 5 beta-6 beta epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3 beta-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3 alpha-5 beta-6 alpha-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed. (C) 2016 Elsevier Ltd. All rights reserved. |
Keywords |
Oxysterol
Mesenchymal stem cell Apoptosis Cell death Mitochondrial hyperpolarization Adipose tissue |
xmlui.dri2xhtml.METS-1.0.item-coverage | Oxford |
Language | English |
Sponsor | Financiadora de Estudos e Projetos (FINEP), Brazil Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil Institute Nacional de Ciencia e Tecnologia - Fluidos Complexos (INCT-FCx), Brazil |
Date | 2017 |
Published in | Journal Of Steroid Biochemistry And Molecular Biology. Oxford, v. 169, p. 164-175, 2017. |
ISSN | 0960-0760 (Sherpa/Romeo, impact factor) |
Publisher | Pergamon-Elsevier Science Ltd |
Extent | 164-175 |
Origin |
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Access rights | Closed access |
Type | Article |
Web of Science ID | WOS:000401391300019 |
URI | https://repositorio.unifesp.br/handle/11600/54604 |
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