Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death

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dc.citation.volume169
dc.contributor.authorSilva, Suelen Feitoza
dc.contributor.authorLevy, Debora
dc.contributor.authorMaria Ruiz, Jorge Luis
dc.contributor.authorde Melo, Thatiana Correa
dc.contributor.authorIsaac, Cesar
dc.contributor.authorFidelis, Maira Luisa [UNIFESP]
dc.contributor.authorRodrigues, Alessandro [UNIFESP]
dc.contributor.authorBydlowski, Sergio Paulo
dc.coverageOxford
dc.date.accessioned2020-07-13T11:53:24Z
dc.date.available2020-07-13T11:53:24Z
dc.date.issued2017
dc.description.abstractMesenchymal stem cells (MSCs) are multipotent cells characterized by self-renewal and cellular differentiation capabilities. Oxysterols comprise a very heterogeneous group derived from cholesterol through enzymatic and non-enzymatic oxidation. Potent effects in cell death processes, including cytoxicity and apoptosis induction, were described in several cell lines. Very little is known about the effects of oxysterols in MSCs. 7-ketocholesterol (7-KC), one of the most important oxysterols, was shown to be cytotoxic to human adipose tissue-derived MSCs. Here, we describe the short-term (24 h) cytotoxic effects of cholestan-3 alpha-5 beta-6 alpha-triol, 3,5 cholestan-7-one, (3 alpha-5 beta-6 alpha)- cholestane-3,6-diol, 7-oxocholest-5-en-3 beta-ylacetate, and 5 beta-6 beta epoxy-cholesterol, on MSCs derived from human adipose tissue. MSCs were isolated from adipose tissue obtained from three young, healthy women. Oxysterols, with the exception of 3,5 cholestan-7-one and 7-oxocholest-5-en-3 beta-yl acetate, led to a complex mode of cell death that include apoptosis, necrosis and autophagy, depending on the type of oxysterol and concentration, being cholestan-3 alpha-5 beta-6 alpha-triol the most effective. Inhibition of proliferation was also promoted by these oxysterols, but no changes in cell cycle were observed. (C) 2016 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Sao Paulo, Sch Med, Lab Genet & Mol Hematol LIM31, Ave Dr Eneas de Carvalho Aguiar,155,1st Floor, BR-05403000 Sao Paulo, SP, Brazil
dc.description.affiliationFed Univ Latin Amer Integrat UNILA, Life & Nat Sci Inst, Ave Tarquinio Joslin dos Santos,1000,Sala 105, BR-85870901 Foz Do Iguacu, Para, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch, Hosp Clin, Cell Culture & Wound Healing Res Lab,Div Plast Su, Ave Dr Arnaldo,455,1st Floor, BR-05403000 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Earth & Exact Sci, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Earth & Exact Sci, Sao Paulo, SP, Brazil
dc.description.provenanceMade available in DSpace on 2020-07-13T11:53:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2017en
dc.description.sourceWeb of Science
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP), Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil
dc.description.sponsorshipInstitute Nacional de Ciencia e Tecnologia - Fluidos Complexos (INCT-FCx), Brazil
dc.format.extent164-175
dc.identifierhttp://dx.doi.org/10.1016/j.jsbmb.2016.04.017
dc.identifier.citationJournal Of Steroid Biochemistry And Molecular Biology. Oxford, v. 169, p. 164-175, 2017.
dc.identifier.doi10.1016/j.jsbmb.2016.04.017
dc.identifier.issn0960-0760
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54604
dc.identifier.wosWOS:000401391300019
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofJournal Of Steroid Biochemistry And Molecular Biology
dc.rightsAcesso restrito
dc.subjectOxysterolen
dc.subjectMesenchymal stem cellen
dc.subjectApoptosisen
dc.subjectCell deathen
dc.subjectMitochondrial hyperpolarizationen
dc.subjectAdipose tissueen
dc.titleOxysterols in adipose tissue-derived mesenchymal stem cell proliferation and deathen
dc.typeArtigo
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