Dissection of the Effects of Quercetin on Mouse Myocardium

Dissection of the Effects of Quercetin on Mouse Myocardium

Author Santos, Michel Santana Google Scholar
Oliveira, Evaleide Diniz [UNIFESP Google Scholar
Santos-Miranda, Artur Google Scholar
Cruz, Jader Santos Google Scholar
Santana Gondim, Antonio Nei Google Scholar
Rodrigues Menezes-Filho, Jose Evaldo Google Scholar
Souza, Diego Santos Google Scholar
Pinho-da-Silva, Leidiane Google Scholar
Guedes Jesus, Itamar Couto Google Scholar
Roman-Campos, Danilo Autor UNIFESP Google Scholar
Guatimosim, Silvia Google Scholar
Lara, Aline Google Scholar
Conde-Garcia, Eduardo Antonio Google Scholar
Lins Vasconcelos, Carla Maria Google Scholar
Abstract Quercetin is a plant flavonoid with several biological activities. This study aimed to describe quercetin effects on contractile and electrophysiological properties of the cardiac muscle as well as on calcium handling. Quercetin elicited positive inotropism that was significantly reduced by propranolol indicating an involvement of the sympathetic nervous system. In cardiomyocytes, 30 M quercetin increased I-Ca,I-L at 0 mV from -0.95 +/- 0.01 A/F to -1.21 +/- 0.08 A/F. The membrane potential at which 50% of the channels are activated (V-0.5) shifted towards more negative potentials from -13.06 +/- 1.52 mV to -19.26 +/- 1.72 mV and did not alter the slope factor. Furthermore, quercetin increased [Ca2+](i) transient by 28% when compared to control. Quercetin accelerated [Ca2+](i) transient decay time, which could be attributed to SERCA activation. In resting cardiomyocytes, quercetin did not change amplitude or frequency of Ca2+ sparks. In isolated heart, quercetin increased heart rate and decreased PRi, QTc and duration of the QRS complex. Thus, we showed that quercetin activates -adrenoceptors, leading to increased L-type Ca2+ current and cell-wide intracellular Ca2+ transient without visible changes in Ca2+ sparks.
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor FAPITEC/SE (Fundacao de Apoio a Pesquisa e a Inovacao Tecnologica do Estado de Sergipe)
CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
FAPEMIG (Fundacao de Amparo a Pesquisa no Estado de Minas Gerais)
FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
UFS (Universidade Federal de Sergipe)
Grant number FAPEMIG: 00460-12
FAPESP: 2014/09861-1
Date 2017
Published in Basic & Clinical Pharmacology & Toxicology. Hoboken, v. 120, n. 6, p. 550-559, 2017.
ISSN 1742-7835 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 550-559
Origin http://dx.doi.org/10.1111/bcpt.12743
Access rights Closed access
Type Article
Web of Science ID WOS:000401146000005
URI https://repositorio.unifesp.br/handle/11600/54430

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