Dissection of the Effects of Quercetin on Mouse Myocardium

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dc.citation.issue6
dc.citation.volume120
dc.contributor.authorSantos, Michel Santana
dc.contributor.authorOliveira, Evaleide Diniz [UNIFESP
dc.contributor.authorSantos-Miranda, Artur
dc.contributor.authorCruz, Jader Santos
dc.contributor.authorSantana Gondim, Antonio Nei
dc.contributor.authorRodrigues Menezes-Filho, Jose Evaldo
dc.contributor.authorSouza, Diego Santos
dc.contributor.authorPinho-da-Silva, Leidiane
dc.contributor.authorGuedes Jesus, Itamar Couto
dc.contributor.authorRoman-Campos, Danilo [UNIFESP]
dc.contributor.authorGuatimosim, Silvia
dc.contributor.authorLara, Aline
dc.contributor.authorConde-Garcia, Eduardo Antonio
dc.contributor.authorLins Vasconcelos, Carla Maria
dc.coverageHoboken
dc.date.accessioned2020-07-13T11:53:10Z
dc.date.available2020-07-13T11:53:10Z
dc.date.issued2017
dc.description.abstractQuercetin is a plant flavonoid with several biological activities. This study aimed to describe quercetin effects on contractile and electrophysiological properties of the cardiac muscle as well as on calcium handling. Quercetin elicited positive inotropism that was significantly reduced by propranolol indicating an involvement of the sympathetic nervous system. In cardiomyocytes, 30 M quercetin increased I-Ca,I-L at 0 mV from -0.95 +/- 0.01 A/F to -1.21 +/- 0.08 A/F. The membrane potential at which 50% of the channels are activated (V-0.5) shifted towards more negative potentials from -13.06 +/- 1.52 mV to -19.26 +/- 1.72 mV and did not alter the slope factor. Furthermore, quercetin increased [Ca2+](i) transient by 28% when compared to control. Quercetin accelerated [Ca2+](i) transient decay time, which could be attributed to SERCA activation. In resting cardiomyocytes, quercetin did not change amplitude or frequency of Ca2+ sparks. In isolated heart, quercetin increased heart rate and decreased PRi, QTc and duration of the QRS complex. Thus, we showed that quercetin activates -adrenoceptors, leading to increased L-type Ca2+ current and cell-wide intracellular Ca2+ transient without visible changes in Ca2+ sparks.en
dc.description.affiliationUniv Fed Sergipe, Lab Heart Biophys, Dept Physiol, Sao Cristovao, SE, Brazil
dc.description.affiliationUniv Fed Sergipe, Dept Physiotherapy, Sao Cristovao, SE, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Excitable Membranes Lab, Dept Biochem & Immunol, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Estado Bahia, Lab Biophys & Pharmacol Heat, Dept Educ, Campus 12, Guanambi, BA, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Cardiomyocyte Intracellular Signaling Lab, Dept Physiol & Biophys, Inst Biol Sci, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPITEC/SE (Fundacao de Apoio a Pesquisa e a Inovacao Tecnologica do Estado de Sergipe)
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.description.sponsorshipFAPEMIG (Fundacao de Amparo a Pesquisa no Estado de Minas Gerais)
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
dc.description.sponsorshipUFS (Universidade Federal de Sergipe)
dc.description.sponsorshipIDFAPEMIG: 00460-12
dc.description.sponsorshipIDFAPESP: 2014/09861-1
dc.format.extent550-559
dc.identifierhttp://dx.doi.org/10.1111/bcpt.12743
dc.identifier.citationBasic & Clinical Pharmacology & Toxicology. Hoboken, v. 120, n. 6, p. 550-559, 2017.
dc.identifier.doi10.1111/bcpt.12743
dc.identifier.issn1742-7835
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54430
dc.identifier.wosWOS:000401146000005
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleDissection of the Effects of Quercetin on Mouse Myocardiumen
dc.typeinfo:eu-repo/semantics/article
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