Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis

Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis

Author Silva, Erick J. R. Autor UNIFESP Google Scholar
Ribeiro, Camilla M. Autor UNIFESP Google Scholar
Mirim, Andre F. M. Autor UNIFESP Google Scholar
Silva, Alan A. S. Google Scholar
Romano, Renata M. Autor UNIFESP Google Scholar
Hallak, Jorge Google Scholar
Avellar, Maria Christina W. Autor UNIFESP Google Scholar
Abstract Bacterial infections are the most prevalent etiological factors of epididymitis, a commonly diagnosed inflammatory disease in the investigation of male infertility factors. The influence of early pathogenic mechanisms at play during bacterial epididymitis on reproductive outcomes is little understood. We report here that experimental epididymitis induced in rats by Gram-negative (LPS) and Gram-positive (LTA) bacterial products resulted in differential patterns of acute inflammation in the cauda epididymis. LPS elicited a strong inflammatory reaction, as reflected by upregulation of levels of mRNA for seven inflammatory mediators (Il1b, Tnf, Il6, Ifng, Il10, Nos2 and Nfkbia), and tissue concentration of six cytokines/chemokines (IL1A, IL1B, IL6, IL10, CXCL2 and CCL2) within the first 24 h post-treatment. Conversely, LTA induced downregulation of one (Nfkbia) and upregulation of six (Il1b, Il6, Nos2, Il4 Il10 and Ptgs1) inflammatory gene transcripts, whereas increased the tissue concentration of three cytokines/chemokines (IL10, CXCL2 and CCL2). The stronger acute inflammatory response induced by LPS correlated with a reduction of epididymal sperm count and transit time that occurred at 1, 7, and 15 days post-treatment. Our study provides evidence that early epididymal inflammatory signaling events to bacterial activators of innate immunity may contribute to the detrimental effects of epididymitis upon male fertility.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
CNPq
Sao Paulo Research Foundation (FAPESP)
Pro-Reitoria de Pesquisa/UNESP
Grant number (CNPq)/CSF/BJT: 401718/2012-3
CNPq: 479546/2013-4, 455450/2014-5, 308349/2010-5
FAPESP: 2010/52711-0, 2015/08227-0
Pro-Reitoria de Pesquisa/UNESP: 557
Date 2018
Published in Scientific Reports. London, v. 8, p. -, 2018.
ISSN 2045-2322 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent -
Origin http://dx.doi.org/10.1038/s41598-017-17944-4
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000419442500040
URI https://repositorio.unifesp.br/handle/11600/54271

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