Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis

Página do item simplificado
dc.citation.volume8
dc.contributor.authorSilva, Erick José Ramo da [UNIFESP]
dc.contributor.authorRibeiro, Camilla Moreira [UNIFESP]
dc.contributor.authorMirim, André Ferradans Mato Neves [UNIFESP]
dc.contributor.authorSilva, Alan Andrew Santos
dc.contributor.authorRomano, Renata Marino [UNIFESP]
dc.contributor.authorHallak, Jorge
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.coverageLondon
dc.date.accessioned2020-07-08T13:09:53Z
dc.date.available2020-07-08T13:09:53Z
dc.date.issued2018
dc.description.abstractBacterial infections are the most prevalent etiological factors of epididymitis, a commonly diagnosed inflammatory disease in the investigation of male infertility factors. The influence of early pathogenic mechanisms at play during bacterial epididymitis on reproductive outcomes is little understood. We report here that experimental epididymitis induced in rats by Gram-negative (LPS) and Gram-positive (LTA) bacterial products resulted in differential patterns of acute inflammation in the cauda epididymis. LPS elicited a strong inflammatory reaction, as reflected by upregulation of levels of mRNA for seven inflammatory mediators (Il1b, Tnf, Il6, Ifng, Il10, Nos2 and Nfkbia), and tissue concentration of six cytokines/chemokines (IL1A, IL1B, IL6, IL10, CXCL2 and CCL2) within the first 24 h post-treatment. Conversely, LTA induced downregulation of one (Nfkbia) and upregulation of six (Il1b, Il6, Nos2, Il4 Il10 and Ptgs1) inflammatory gene transcripts, whereas increased the tissue concentration of three cytokines/chemokines (IL10, CXCL2 and CCL2). The stronger acute inflammatory response induced by LPS correlated with a reduction of epididymal sperm count and transit time that occurred at 1, 7, and 15 days post-treatment. Our study provides evidence that early epididymal inflammatory signaling events to bacterial activators of innate immunity may contribute to the detrimental effects of epididymitis upon male fertility.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Sect Expt Endocrinol, Dept Pharmacol, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Pharmacol, Inst Biosci Botucatu, Botucatu, SP, Brazil
dc.description.affiliationSci & Innovat Ctr Androl, Androsci, BR-03178200 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch, Hosp Clin, Reprod Toxicol Unity,Dept Pathol, BR-01246903 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch, Hosp Clin, Div Urol, BR-01246903 Sao Paulo, SP, Brazil
dc.description.affiliationState Univ Centro Oeste, Dept Pharm, BR-85040080 Guarapuava, PR, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Sect Expt Endocrinol, Dept Pharmacol, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipPro-Reitoria de Pesquisa/UNESPpt
dc.description.sponsorshipIDCNPq: 401718/2012-3pt
dc.description.sponsorshipIDCNPq: 479546/2013-4pt
dc.description.sponsorshipIDCNPq: 455450/2014-5pt
dc.description.sponsorshipIDCNPq: 308349/2010-5pt
dc.description.sponsorshipIDFAPESP: 2010/52711-0pt
dc.description.sponsorshipIDFAPESP: 2015/08227-0pt
dc.description.sponsorshipIDPro-Reitoria de Pesquisa/UNESP: 557pt
dc.format.extent-
dc.identifierhttps://dx.doi.org/10.1038/s41598-017-17944-4
dc.identifier.citationScientific Reports. London, v. 8, p. -, 2018.
dc.identifier.doi10.1038/s41598-017-17944-4
dc.identifier.fileWOS000419442500040.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54271
dc.identifier.wosWOS:000419442500040
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleLipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitisen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000419442500040.pdf
Tamanho:
2.68 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos