Technical challenges of working with extracellular vesicles
Arquivos
Data
2018
Autores
Ramirez, Marcel Ivan
Amorim, Maria G.
Gadelha, Catarina
Milic, Ivana
Welsh, Joshua A.
Freitas, Vanessa Morais
Nawaz, Muhammad
Akbar, Naveed
Couch, Yvonne
Makin, Laura
Orientadores
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nano-sized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
Descrição
Citação
Nanoscale. Cambridge, v. 10, n. 3, p. 881-906, 2018.