Nutritional shortage augments cisplatin-effects on murine melanoma cells

Nutritional shortage augments cisplatin-effects on murine melanoma cells

Author Antunes, F. Autor UNIFESP Google Scholar
Pereira, G. J. Autor UNIFESP Google Scholar
Jasiulionis, M. G. Autor UNIFESP Google Scholar
Bincoletto, C. Autor UNIFESP Google Scholar
Smaili, S. S. Autor UNIFESP Google Scholar
Abstract Melanoma incidence increases every year worldwide and is responsible for 80% of skin cancer deaths. Due to its metastatic potential and resistance to almost any treatments such as chemo, radio, immune and targeted-therapy, the patients still have a poor prognosis, especially at metastatic stage. Considering that, it is crucial to find new therapeutic approaches to overcome melanoma resistance. Here we investigated the effect of cisplatin (CDDP), one of the chemotherapeutic agents used for melanoma treatment, in association with nutritional deprivation in murine melanoma cell lines. Cell death and autophagy were evaluated after the treatment with cisplatin, nutritional deprivation and its association using an in vitro model of murine melanocytes malignant transformation to metastatic melanoma. Our results showed that nutritional deprivation augmented cell death induced by cisplatin in melanoma cells, especially at the metastatic subtype, with slight effects on melanocytes. Mechanistic studies revealed that although autophagy was present at high levels in basal conditions in melanoma cells, was not essential for cell death process that involved mitochondrial damage, reactive oxygen species production and possible glycolysis inhibition. In conclusion, nutritional shortage in combination with chemotherapeutic drugs as cisplatin can be a valuable new therapeutic strategy to overcome melanoma resistance.
Keywords Melanoma
Autophagy
Chemosensitization
Cisplatin
Nutrient deprivation
Starvation
xmlui.dri2xhtml.METS-1.0.item-coverage Clare
Language English
Sponsor FAPESP: 13/20073-2, 12/51215-4
CNPq: 401236/2014-5
FAPESP: 13/20073-2, 12/51215-4
CNPq: 401236/2014-5
Grant number (FAPESP) [13/20073-2, 12/51215-4]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401236/2014-5]
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/20073-2, 12/51215-4]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401236/2014-5]
Date 2018
Published in Chemico-Biological Interactions. Clare, v. 281, p. 89-97, 2018.
ISSN 0009-2797 (Sherpa/Romeo, impact factor)
Publisher Elsevier Ireland Ltd
Extent 89-97
Origin http://dx.doi.org/10.1016/j.cbi.2017.12.027
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000422742700010
URI https://repositorio.unifesp.br/handle/11600/54233

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