Nutritional shortage augments cisplatin-effects on murine melanoma cells

Página do item simplificado
dc.citation.volume281
dc.contributor.authorAntunes, F. [UNIFESP]
dc.contributor.authorPereira, G. J. [UNIFESP]
dc.contributor.authorJasiulionis, M. G. [UNIFESP]
dc.contributor.authorBincoletto, C. [UNIFESP]
dc.contributor.authorSmaili, S. S. [UNIFESP]
dc.coverageClare
dc.date.accessioned2020-07-08T13:09:50Z
dc.date.available2020-07-08T13:09:50Z
dc.date.issued2018
dc.description.abstractMelanoma incidence increases every year worldwide and is responsible for 80% of skin cancer deaths. Due to its metastatic potential and resistance to almost any treatments such as chemo, radio, immune and targeted-therapy, the patients still have a poor prognosis, especially at metastatic stage. Considering that, it is crucial to find new therapeutic approaches to overcome melanoma resistance. Here we investigated the effect of cisplatin (CDDP), one of the chemotherapeutic agents used for melanoma treatment, in association with nutritional deprivation in murine melanoma cell lines. Cell death and autophagy were evaluated after the treatment with cisplatin, nutritional deprivation and its association using an in vitro model of murine melanocytes malignant transformation to metastatic melanoma. Our results showed that nutritional deprivation augmented cell death induced by cisplatin in melanoma cells, especially at the metastatic subtype, with slight effects on melanocytes. Mechanistic studies revealed that although autophagy was present at high levels in basal conditions in melanoma cells, was not essential for cell death process that involved mitochondrial damage, reactive oxygen species production and possible glycolysis inhibition. In conclusion, nutritional shortage in combination with chemotherapeutic drugs as cisplatin can be a valuable new therapeutic strategy to overcome melanoma resistance.en
dc.description.affiliationUniv Fed Sao Paulo, EPM, Dept Pharmacol, UNIFESP, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, EPM, Dept Pharmacol, UNIFESP, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP: 13/20073-2, 12/51215-4
dc.description.sponsorshipCNPq: 401236/2014-5
dc.description.sponsorshipFAPESP: 13/20073-2, 12/51215-4
dc.description.sponsorshipCNPq: 401236/2014-5
dc.description.sponsorshipID(FAPESP) [13/20073-2, 12/51215-4]
dc.description.sponsorshipIDConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401236/2014-5]
dc.description.sponsorshipIDFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/20073-2, 12/51215-4]
dc.description.sponsorshipIDConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [401236/2014-5]
dc.format.extent89-97
dc.identifierhttp://dx.doi.org/10.1016/j.cbi.2017.12.027
dc.identifier.citationChemico-Biological Interactions. Clare, v. 281, p. 89-97, 2018.
dc.identifier.doi10.1016/j.cbi.2017.12.027
dc.identifier.issn0009-2797
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54233
dc.identifier.wosWOS:000422742700010
dc.language.isoeng
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofChemico-Biological Interactions
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMelanomaen
dc.subjectAutophagyen
dc.subjectChemosensitizationen
dc.subjectCisplatinen
dc.subjectNutrient deprivationen
dc.subjectStarvationen
dc.titleNutritional shortage augments cisplatin-effects on murine melanoma cellsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos