Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)

Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)

Author Pereira, Kassia M. F. Autor UNIFESP Google Scholar
Grecco, Simone S. Google Scholar
Figueiredo, Carlos R. Autor UNIFESP Google Scholar
Hosomi, Jorge K. Autor UNIFESP Google Scholar
Nakamura, Mari U. Autor UNIFESP Google Scholar
Lago, Joao Henrique G. Google Scholar
Abstract Kalanchoe pinnata (Crassulaceae) is a medicinal plant distributed in several areas of the World. Their leaves have been used, in folk medicine, for anxiety and sleep disorders among other proposes. Chemically, this plant is composed by organic acids, flavonoids, bufadienolides, triterpenoids and some ubiquitous compounds. In this study, the fresh leaves of K. pinnata were extracted in an accelerated solvent extraction (ASE) system using H2O (cold and hot) as well as with EtOH:H2O 1:1. The obtained crude extracts were analyzed by HPLC-UV-MS. As a result, were identified organic acids and flavonoids, these being quercetin-3-O-alpha-L-arabinopyranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-arabinopiranosyl-(1 -> 2)-alpha-L-rhamnopyranoside, quercitrin, and trans-p-coumaryl glutaric acid the main compounds in the studied extracts. Obtained results revealed the predominance of flavonoids in extracts prepared using hot H2O (70.26 +/- 2.19%) or EtOH:H2O 1:1 (74.65 +/- 5.39%) while a reduction in the amount of these compounds was observed in the extracts prepared using cold H2O (54.41 +/- 2.93%). Additionally, crude extracts from leaves of K. pinnata extracted with hot H2O and EtOH:H2O 1:1 showed in vitro cytotoxicity on five tested cancer cell lines: murine melanoma (B16F10), human melanoma (A2058), human cervical carcinoma (HeLa), human leukemia (HL-60), and human glioblastoma (U87-MG), with IC50 values in the range of 10.6 +/- 2.5 - 91.3 +/- 7.2 mu g/mL. On the other hand, the extract prepared using cold H2O displayed reduced potential. These crude extracts were also tested against human foreskin fibroblasts (non-tumorigenic cells) showing IC50 values ranging from 79.5 to 90.2 mu g/mL. In conclusion, our results showed the impact that different extraction methods have on the chemical composition and pharmacological activity, which are notable for the anticancer activity associated to flavonoids.
Keywords Kalanchoe pinnata
Accelerated solvent extraction (ASE)
Chemical composition
Flavonoids
Anticancer activity
xmlui.dri2xhtml.METS-1.0.item-coverage Westerville
Language English
Sponsor FAPESP
CNPq
Date 2018
Published in Natural Product Communications. Westerville, v. 13, n. 2, p. 163-166, 2018.
ISSN 1934-578X (Sherpa/Romeo, impact factor)
Publisher Natural Products Inc
Extent 163-166
Origin https://doi.org/10.1177/1934578X1801300213
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000433651700013
URI https://repositorio.unifesp.br/handle/11600/54148

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